Product nameAnti-DAK antibody
DescriptionRabbit polyclonal to DAK
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Recombinant fragment, corresponding to a region within amino acids 273-548 of Human DAK
- HepG2 and Raji whole cell lysates, Human lung SCC, HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 59 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionCatalyzes both the phosphorylation of dihydroxyacetone and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate.
Sequence similaritiesBelongs to the dihydroxyacetone kinase (DAK) family.
Contains 1 DhaK domain.
Contains 1 DhaL domain.
- Information by UniProt
- ATP dependent dihydroxyacetone kinase antibody
- Bifunctional ATP dependent dihydroxyacetone kinase/FAD AMP lyase (cyclizing) antibody
- DAK antibody
All lanes : Anti-DAK antibody (ab137623) at 1/500 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin embedded Human lung SCC labelling DAK with ab137623 antibody at a dilution of 1/100.
Immunofluorescent analysis of methanol-fixed HeLa cells labelling DAK with ab137623 at 1/100 dilution. Picture at the bottom costained with Hoechst 33342.
ab137623 has not yet been referenced specifically in any publications.