Overview

  • Product name

    Anti-DAK antibody
  • Description

    Rabbit polyclonal to DAK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 273-548 of Human DAK

  • Positive control

    • HepG2 and Raji whole cell lysates, Human lung SCC, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab137623 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 59 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes both the phosphorylation of dihydroxyacetone and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate.
  • Sequence similarities

    Belongs to the dihydroxyacetone kinase (DAK) family.
    Contains 1 DhaK domain.
    Contains 1 DhaL domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • ATP dependent dihydroxyacetone kinase antibody
    • Bifunctional ATP dependent dihydroxyacetone kinase/FAD AMP lyase (cyclizing) antibody
    • DAK antibody
    • DHA kinase antibody
    • Dha kinase/FMN cyclase antibody
    • DHAK_HUMAN antibody
    • Dihydroxyacetone kinase antibody
    • Dihydroxyacetone kinase 2 antibody
    • Dihydroxyacetone kinase 2 homolog (S. cerevisiae) antibody
    • Dihydroxyacetone kinase 2 homolog (yeast) antibody
    • FAD AMP lyase (cyclic FMN forming) antibody
    • FAD AMP lyase (cyclizing) antibody
    • FAD-AMP lyase (cyclic FMN forming) antibody
    • FAD-AMP lyase (cyclizing) antibody
    • FMN cyclase antibody
    • Glycerone kinase antibody
    see all

Images

  • All lanes : Anti-DAK antibody (ab137623) at 1/500 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 59 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human lung SCC labelling DAK with ab137623 antibody at a dilution of 1/100.
  • Immunofluorescent analysis of methanol-fixed HeLa cells labelling DAK with ab137623 at 1/100 dilution. Picture at the bottom costained with Hoechst 33342.

References

ab137623 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137623.
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