Overview

  • Product name
    Anti-DAP1 antibody [E59]
    See all DAP1 primary antibodies
  • Description
    Rabbit monoclonal [E59] to DAP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human DAP1 aa 50-150 (C terminal). The exact sequence is proprietary.

  • Positive control
    • NIH3T3 cell lysate, Hela cell lysate and human stomach carcinoma
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab32056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 11 kDa.
IHC-P 1/100 - 1/250.
IP 1/50.
ICC/IF 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    Images

    • Immunofluorescent analysis of DAP1 expression in HeLa cells using 1/250 ab32056.
    • Anti-DAP1 antibody [E59] (ab32056) at 1/10000 dilution + NIH3T3 cell lysate

      Observed band size: 11 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.

    • ab32056, at a 1/100 dilution, staining human stomach carcinoma by immunohistochemistry, Paraffin embedded tissue.

    References

    ab32056 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your reply. Unfortunately, I am not an expert on DAP1 so I don't have any further information about what effect the acetylserine has in the cell. According to the uniprot database, the reference PMID 16602700 contains additional information about this specific acetylation. In general, post translational modifications of proteins can often lead to changes in their subcellular localization or expression level. I am sorry I could not be more helpful, please let me know if you have any further questions.

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    Answer

    Thank you for contacting us. We do not currently sell an antibody to DAP1 that is specific for the N-acetylserine modification. If you are looking to detect this modification, you may be able to perform an IP for the total DAP1 protein using ab32056 and perform a western blot on the IP product using an antibody designed to detect all acetylations such as ab193. I hope this helps, please let me know if you need any additional information.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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