Overview

  • Product name

  • Description

    Rabbit polyclonal to DAP12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep, Cow, Dog
  • Immunogen

    Synthetic peptide corresponding to Human DAP12 aa 50 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab107906)

  • Positive control

    • This antibody gave a positive signal in the follwoing lysates: Lung (Human) Tissue Lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.
  • Tissue specificity

    Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.
  • Involvement in disease

    Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • Sequence similarities

    Belongs to the TYROBP family.
  • Post-translational
    modifications

    Tyrosine phosphorylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DAP 12 antibody
    • DAP12 antibody
    • DNAX activation protein 12 antibody
    • DNAX-activation protein 12 antibody
    • KAR-associated protein antibody
    • KARAP antibody
    • Killer activating receptor associated protein antibody
    • Killer-activating receptor-associated protein antibody
    • PLOSL antibody
    • TYOBP_HUMAN antibody
    • TYRO protein tyrosine kinase binding protein antibody
    • TYRO protein tyrosine kinase-binding protein antibody
    • TYROBP antibody
    see all

Images

  • Anti-DAP12 antibody (ab93846) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 12 kDa
    Observed band size: 12 kDa
    Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes


    The DAP12 protein sequence contains a 21-residue signal sequence at the N-terminus. Cleavage of this peptide may account for the doublet observed.
  • ICC/IF image of ab93846 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93846, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab93846, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293 and HepG2 cells at 10µg/ml.

References

This product has been referenced in:

  • Raha AA  et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). IHC ; Mouse . Read more (PubMed: 27662313) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Mouse brain tissues)
Specification
Mouse brain tissues
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Paraformaldehyde

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 06 2013

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