Overview

  • Product name

    Anti-DAP12 antibody - C-terminal
    See all DAP12 primary antibodies
  • Description

    Rabbit polyclonal to DAP12 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human DAP12 aa 85-113 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: O43914

  • Positive control

    • WB: HepG2 cell lysate. IHC-P: Human lung tissue. Flow Cyt: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 12 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.
  • Tissue specificity

    Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.
  • Involvement in disease

    Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • Sequence similarities

    Belongs to the TYROBP family.
  • Post-translational
    modifications

    Tyrosine phosphorylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • DAP 12 antibody
    • DAP12 antibody
    • DNAX activation protein 12 antibody
    • DNAX-activation protein 12 antibody
    • KAR-associated protein antibody
    • KARAP antibody
    • Killer activating receptor associated protein antibody
    • Killer-activating receptor-associated protein antibody
    • PLOSL antibody
    • TYOBP_HUMAN antibody
    • TYRO protein tyrosine kinase binding protein antibody
    • TYRO protein tyrosine kinase-binding protein antibody
    • TYROBP antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human lung tissue stained for DAP12 using ab230433 at 1/100 dilution in immunohistochemical analysis.

  • Anti-DAP12 antibody - C-terminal (ab230433) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 35 µg

    Predicted band size: 12 kDa

  • Flow cytometric analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells labeling DAP12 with ab230433 at 1/10 dilution (green) compared with a negative control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used.

References

ab230433 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab230433.
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