Key features and details
- Rabbit polyclonal to DAP12 - C-terminal
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DAP12 antibody - C-terminal
See all DAP12 primary antibodies
DescriptionRabbit polyclonal to DAP12 - C-terminal
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Cow
Synthetic peptide within Human DAP12 aa 85-113 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O43914
- WB: HepG2 cell lysate. IHC-P: Human lung tissue. Flow Cyt: HepG2 cells.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 12 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.|
FunctionNon-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.
Tissue specificityExpressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.
Involvement in diseaseDefects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
Sequence similaritiesBelongs to the TYROBP family.
- Information by UniProt
- DAP 12 antibody
- DAP12 antibody
- DNAX activation protein 12 antibody
Formalin-fixed, paraffin-embedded human lung tissue stained for DAP12 using ab230433 at 1/100 dilution in immunohistochemical analysis.
Anti-DAP12 antibody - C-terminal (ab230433) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 35 µg
Predicted band size: 12 kDa
Flow cytometric analysis of HepG2 (human liver hepatocellular carcinoma cell line) cells labeling DAP12 with ab230433 at 1/10 dilution (green) compared with a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230433 has not yet been referenced specifically in any publications.