RecombinantRabMAb

Recombinant Anti-DAP12 antibody [EPR5173] (ab124834)

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Flow Cytometry (Intracellular) - Anti-DAP12 antibody [EPR5173] (ab124834)
  • Western blot - Anti-DAP12 antibody [EPR5173] (ab124834)
  • Immunocytochemistry - Anti-DAP12 antibody [EPR5173] (ab124834)
  • OI-RD Scanning - Anti-DAP12 antibody [EPR5173] (ab124834)
  • Anti-DAP12 antibody [EPR5173] (ab124834)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5173] to DAP12
  • Suitable for: Flow Cyt (Intra), ICC, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-DAP12 antibody [EPR5173]
    See all DAP12 primary antibodies

  • Description

    Rabbit monoclonal [EPR5173] to DAP12

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human DAP12 aa 50-150. The exact sequence is proprietary.
    Database link: O43914

  • Positive control

    • Peripheral blood leukocytes, Human placenta and peripheral blood mononuclear cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.86 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Purification notes

    Not purified; provided in cell supernatant and storage buffer.

  • Clonality

    Monoclonal

  • Clone number

    EPR5173

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab124834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC
1/250 - 1/500.
WB
1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
Notes
Flow Cyt (Intra)
1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC
1/250 - 1/500.
WB
1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
Application notes
Is unsuitable for IHC-P or IP.

Target

  • Function

    Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.

  • Tissue specificity

    Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.

  • Involvement in disease

    Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.

  • Sequence similarities

    Belongs to the TYROBP family.

  • Post-translational
    modifications

    Tyrosine phosphorylated.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • DAP 12 antibody
      • DAP12 antibody
      • DNAX activation protein 12 antibody
      • DNAX-activation protein 12 antibody
      • KAR-associated protein antibody
      • KARAP antibody
      • Killer activating receptor associated protein antibody
      • Killer-activating receptor-associated protein antibody
      • PLOSL antibody
      • TYOBP_HUMAN antibody
      • TYRO protein tyrosine kinase binding protein antibody
      • TYRO protein tyrosine kinase-binding protein antibody
      • TYROBP antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Anti-DAP12 antibody [EPR5173] (ab124834)
      Flow Cytometry (Intracellular) - Anti-DAP12 antibody [EPR5173] (ab124834)

      Overlay histogram showing THP1 cells stained with ab124834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124834, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

       

    • Western blot - Anti-DAP12 antibody [EPR5173] (ab124834)
      Western blot - Anti-DAP12 antibody [EPR5173] (ab124834)
      All lanes : Anti-DAP12 antibody [EPR5173] (ab124834) at 1/1000 dilution

      Lane 1 : Peripheral blood leukocytes lysates
      Lane 2 : Human placenta lysates
      Lane 3 : Peripheral blood mononuclear lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 12 kDa

    • Immunocytochemistry - Anti-DAP12 antibody [EPR5173] (ab124834)
      Immunocytochemistry - Anti-DAP12 antibody [EPR5173] (ab124834)
      Immunocytochemistry analysis of Jurkat (human T cell leukemia T lymphocyte) labeling DAP12 with purified ab124834 at 1/100 dilution (10 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.7 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
      Negative control: PBS instead of the primary antibody.
    • OI-RD Scanning - Anti-DAP12 antibody [EPR5173] (ab124834)
      OI-RD Scanning - Anti-DAP12 antibody [EPR5173] (ab124834)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-DAP12 antibody [EPR5173] (ab124834)
      Anti-DAP12 antibody [EPR5173] (ab124834)

    References (4)

    Publishing research using ab124834? Please let us know so that we can cite the reference in this datasheet.

    ab124834 has been referenced in 4 publications.

    • Liu M  et al. Bioinformatics analysis indicates that microRNA-628-5p overexpression may alleviate Alzheimer's disease by targeting TYROBP. Mol Med Rep 23:N/A (2021). PubMed: 33313948
    • Grubman A  et al. Transcriptional signature in microglia associated with Aß plaque phagocytosis. Nat Commun 12:3015 (2021). PubMed: 34021136
    • Duggan SP  et al. siRNA Library Screening Identifies a Druggable Immune-Signature Driving Esophageal Adenocarcinoma Cell Growth. Cell Mol Gastroenterol Hepatol 5:569-590 (2018). PubMed: 29930979
    • Lue LF  et al. TREM2 Protein Expression Changes Correlate with Alzheimer's Disease Neurodegenerative Pathologies in Post-Mortem Temporal Cortices. Brain Pathol 25:469-80 (2015). IHC-FrFl ; Human . PubMed: 25186950

    Customer reviews and Q&As

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    Immunohistochemistry (Frozen sections) abreview for Anti-DAP12 antibody [EPR5173]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Human Tissue sections (Blood vessels)
    Specification
    Blood vessels
    Permeabilization
    Yes - 0.1% TritonX in 0.1% PBS
    Fixative
    Formaldehyde
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Aug 06 2013

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