Product nameAnti-DAP12 antibody [EPR5173]
See all DAP12 primary antibodies
DescriptionRabbit monoclonal [EPR5173] to DAP12
Tested applicationsSuitable for: IHC-Fr, WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human DAP12 aa 50-150. The exact sequence is proprietary.
Database link: O43914
- Peripheral blood leukocytes, Human placenta and peripheral blood mononuclear cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.86 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Purification notesNot purified; provided in cell supernatant and storage buffer.
Our Abpromise guarantee covers the use of ab124834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 12 kDa.|
|ICC/IF||1/250 - 1/500.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionNon-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.
Tissue specificityExpressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.
Involvement in diseaseDefects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
Sequence similaritiesBelongs to the TYROBP family.
- Information by UniProt
- DAP 12 antibody
- DAP12 antibody
- DNAX activation protein 12 antibody
IHC-Fr image of human blood vessels stained with Ab124834 (1/500). The fixed brain section were permeabilise in 0.1% PBS-Triton X for 1h. The sections were incubated with 10% donkey serum for 1 h at 24°C to block non-specific protein-protein interactions. The sections were then incubated with Ab124834 used at 1/500 dilution, overnight at 4°C. The secondary antibody was Alexa Fluor® 488 donkey anti-rabbit used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Overlay histogram showing THP1 cells stained with ab124834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124834, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
All lanes : Anti-DAP12 antibody [EPR5173] (ab124834) at 1/1000 dilution
Lane 1 : Peripheral blood leukocytes lysates
Lane 2 : Human placenta lysates
Lane 3 : Peripheral blood mononuclear lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 12 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Duggan SP et al. siRNA Library Screening Identifies a Druggable Immune-Signature Driving Esophageal Adenocarcinoma Cell Growth. Cell Mol Gastroenterol Hepatol 5:569-590 (2018). Read more (PubMed: 29930979) »
- Lue LF et al. TREM2 Protein Expression Changes Correlate with Alzheimer's Disease Neurodegenerative Pathologies in Post-Mortem Temporal Cortices. Brain Pathol 25:469-80 (2015). IHC-FrFl ; Human . Read more (PubMed: 25186950) »