Recombinant
RabMAb

Recombinant Anti-DAP12 antibody [EPR5173] (ab124834)

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Overview

  • Product name

    Anti-DAP12 antibody [EPR5173]
    See all DAP12 primary antibodies

  • Description

    Rabbit monoclonal [EPR5173] to DAP12

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human DAP12 aa 50-150. The exact sequence is proprietary.
    Database link: O43914

  • Positive control

    • Peripheral blood leukocytes, Human placenta and peripheral blood mononuclear cell lysates.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 1.86 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Purification notes

    Not purified; provided in cell supernatant and storage buffer.

  • Clonality

    Monoclonal

  • Clone number

    EPR5173

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/500.
WB 1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.

    • Tissue specificity

      Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.

    • Involvement in disease

      Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.

    • Sequence similarities

      Belongs to the TYROBP family.

    • Post-translational
      modifications

      Tyrosine phosphorylated.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • DAP 12 antibody
        • DAP12 antibody
        • DNAX activation protein 12 antibody
        • DNAX-activation protein 12 antibody
        • KAR-associated protein antibody
        • KARAP antibody
        • Killer activating receptor associated protein antibody
        • Killer-activating receptor-associated protein antibody
        • PLOSL antibody
        • TYOBP_HUMAN antibody
        • TYRO protein tyrosine kinase binding protein antibody
        • TYRO protein tyrosine kinase-binding protein antibody
        • TYROBP antibody
        see all

      Images

      • Immunohistochemistry (Frozen sections) - Anti-DAP12 antibody [EPR5173] (ab124834)
        Immunohistochemistry (Frozen sections) - Anti-DAP12 antibody [EPR5173] (ab124834)This image is courtesy of an abreview submitted by Dr. Ruma Raha-Chowdhury, Cambridge University, United Kingdom.

        IHC-Fr image of human blood vessels stained with Ab124834 (1/500). The fixed brain section were permeabilise in 0.1% PBS-Triton X for 1h. The sections were incubated with 10% donkey serum for 1 h at 24°C to block non-specific protein-protein interactions. The sections were then incubated with Ab124834 used at 1/500 dilution, overnight at 4°C. The secondary antibody was Alexa Fluor® 488 donkey anti-rabbit used at a 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

        See Abreview

      • Flow Cytometry - Anti-DAP12 antibody [EPR5173] (ab124834)
        Flow Cytometry - Anti-DAP12 antibody [EPR5173] (ab124834)
        Overlay histogram showing THP1 cells stained with ab124834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124834, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
      • Western blot - Anti-DAP12 antibody [EPR5173] (ab124834)
        Western blot - Anti-DAP12 antibody [EPR5173] (ab124834)
        All lanes : Anti-DAP12 antibody [EPR5173] (ab124834) at 1/1000 dilution

        Lane 1 : Peripheral blood leukocytes lysates
        Lane 2 : Human placenta lysates
        Lane 3 : Peripheral blood mononuclear lysates

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 12 kDa

      • Other - Anti-DAP12 antibody [EPR5173] (ab124834)
        Other - Anti-DAP12 antibody [EPR5173] (ab124834)
        Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD

      References

      This product has been referenced in:

      • Duggan SP  et al. siRNA Library Screening Identifies a Druggable Immune-Signature Driving Esophageal Adenocarcinoma Cell Growth. Cell Mol Gastroenterol Hepatol 5:569-590 (2018). Read more (PubMed: 29930979) »
      • Lue LF  et al. TREM2 Protein Expression Changes Correlate with Alzheimer's Disease Neurodegenerative Pathologies in Post-Mortem Temporal Cortices. Brain Pathol 25:469-80 (2015). IHC-FrFl ; Human . Read more (PubMed: 25186950) »
      See all 2 Publications for this product

      Publishing research using ab124834? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Immunohistochemistry (Frozen sections) abreview for Anti-DAP12 antibody [EPR5173]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
      Sample
      Human Tissue sections (Blood vessels)
      Specification
      Blood vessels
      Permeabilization
      Yes - 0.1% TritonX in 0.1% PBS
      Fixative
      Formaldehyde
      Read More

      Dr. Ruma Raha-Chowdhury

      Verified customer

      Submitted Aug 06 2013

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