Recombinant Anti-DAP12 antibody [EPR5173] (ab124834)
![Flow Cytometry - Anti-DAP12 antibody [EPR5173] (ab124834)](https://www.abcam.com/ps/products/124/ab124834/Images/ab124834-210407-anti-dap12-antibody-epr5173-flow-cytometry.jpg "Flow Cytometry - Anti-DAP12 antibody [EPR5173] (ab124834)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5173] to DAP12
- Suitable for: ICC, WB, Flow Cyt
- Reacts with: Human
Overview
-
Product name
Anti-DAP12 antibody [EPR5173]
See all DAP12 primary antibodies -
Description
Rabbit monoclonal [EPR5173] to DAP12 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC HumanWB Human
-
Immunogen
Synthetic peptide within Human DAP12 aa 50-150. The exact sequence is proprietary.
Database link: O43914 -
Positive control
- Peripheral blood leukocytes, Human placenta and peripheral blood mononuclear cell lysates.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.86 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Not purified; provided in cell supernatant and storage buffer. -
Clonality
Monoclonal -
Clone number
EPR5173 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| ICC |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| ICC |
1/250 - 1/500.
|
|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 12 kDa.
|
|
| Flow Cyt |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC
1/250 - 1/500. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 12 kDa. |
|
Flow Cyt
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
-
Function
Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation. -
Tissue specificity
Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum. -
Involvement in disease
Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
Sequence similarities
Belongs to the TYROBP family. -
Post-translational
modificationsTyrosine phosphorylated. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 7305 Human
- Omim: 604142 Human
- SwissProt: O43914 Human
- Unigene: 515369 Human
-
Alternative names
- DAP 12 antibody
- DAP12 antibody
- DNAX activation protein 12 antibody
see all
Images
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Flow Cytometry - Anti-DAP12 antibody [EPR5173] (ab124834)Overlay histogram showing THP1 cells stained with ab124834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124834, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Western blot - Anti-DAP12 antibody [EPR5173] (ab124834)](https://www.abcam.com/ps/products/124/ab124834/Images/ab124834-138786-anti-dap12-antibody-epr5173-western-blot.jpg)
Western blot - Anti-DAP12 antibody [EPR5173] (ab124834)All lanes : Anti-DAP12 antibody [EPR5173] (ab124834) at 1/1000 dilution
Lane 1 : Peripheral blood leukocytes lysates
Lane 2 : Human placenta lysates
Lane 3 : Peripheral blood mononuclear lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 12 kDa -
![Immunocytochemistry - Anti-DAP12 antibody [EPR5173] (ab124834)](https://www.abcam.com/ps/products/124/ab124834/Images/ab124834-5-anti-dap12-antibody-epr5173-immunocytochemistry-jurkat-human.jpg)
Immunocytochemistry - Anti-DAP12 antibody [EPR5173] (ab124834)Immunocytochemistry analysis of Jurkat (human T cell leukemia T lymphocyte) labeling DAP12 with purified ab124834 at 1/100 dilution (10 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.7 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody. -
![OI-RD Scanning - Anti-DAP12 antibody [EPR5173] (ab124834)](https://www.abcam.com/ps/products/124/ab124834/Images/ab124834-138785-anti-dap12-antibody-epr5173.jpg)
OI-RD Scanning - Anti-DAP12 antibody [EPR5173] (ab124834) -
![Anti-DAP12 antibody [EPR5173] (ab124834)](https://www.abcam.com/ps/products/124/ab124834/Images/ab124834-6-benefits-of-recombinant-antibodies.png)
Anti-DAP12 antibody [EPR5173] (ab124834)
Protocols
Datasheets and documents
References (2)
ab124834 has been referenced in 2 publications.
- Duggan SP et al. siRNA Library Screening Identifies a Druggable Immune-Signature Driving Esophageal Adenocarcinoma Cell Growth. Cell Mol Gastroenterol Hepatol 5:569-590 (2018). PubMed: 29930979
- Lue LF et al. TREM2 Protein Expression Changes Correlate with Alzheimer's Disease Neurodegenerative Pathologies in Post-Mortem Temporal Cortices. Brain Pathol 25:469-80 (2015). IHC-FrFl ; Human . PubMed: 25186950
