RecombinantRabMAb

Recombinant Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)

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Flow Cytometry - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
  • Western blot - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
  • Immunocytochemistry - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
  • OI-RD Scanning - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
  • Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5173] to DAP12 - BSA and Azide free
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-DAP12 antibody [EPR5173] - BSA and Azide free
    See all DAP12 primary antibodies

  • Description

    Rabbit monoclonal [EPR5173] to DAP12 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human DAP12 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: O43914

  • Positive control

    • ICC: Jurkat cells

  • General notes

    ab246001 is the carrier-free version of ab124834 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab246001 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 1.86 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5173

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab246001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.

    • Tissue specificity

      Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.

    • Involvement in disease

      Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.

    • Sequence similarities

      Belongs to the TYROBP family.

    • Post-translational
      modifications

      Tyrosine phosphorylated.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • DAP 12 antibody
        • DAP12 antibody
        • DNAX activation protein 12 antibody
        • DNAX-activation protein 12 antibody
        • KAR-associated protein antibody
        • KARAP antibody
        • Killer activating receptor associated protein antibody
        • Killer-activating receptor-associated protein antibody
        • PLOSL antibody
        • TYOBP_HUMAN antibody
        • TYRO protein tyrosine kinase binding protein antibody
        • TYRO protein tyrosine kinase-binding protein antibody
        • TYROBP antibody
        see all

      Images

      • Flow Cytometry - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
        Flow Cytometry - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
        This data was developed using ab124834, the same antibody clone in a different buffer formulation.Overlay histogram showing THP1 cells stained with ab124834 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% human serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab124834, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
      • Western blot - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
        Western blot - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
        All lanes : Anti-DAP12 antibody [EPR5173] (ab124834) at 1/1000 dilution

        Lane 1 : Peripheral blood leukocytes lysates
        Lane 2 : Human placenta lysates
        Lane 3 : Peripheral blood mononuclear lysates

        Lysates/proteins at 10 µg per lane.


        This data was developed using ab124834, the same antibody clone in a different buffer formulation.

      • Immunocytochemistry - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
        Immunocytochemistry - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
        This data was developed using the same antibody clone in a different buffer formulation (ab124834).
        Immunocytochemistry analysis of Jurkat (human T cell leukemia T lymphocyte) labeling DAP12 with purified ab124834 at 1/100 dilution (10 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.7 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
        Negative control: PBS instead of the primary antibody.
      • OI-RD Scanning - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
        OI-RD Scanning - Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
        This data was developed using ab124834, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)
        Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab246001? Please let us know so that we can cite the reference in this datasheet.

      ab246001 has not yet been referenced specifically in any publications.

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