Recombinant
RabMAb

Recombinant Anti-DAP12 antibody [EPR5173] - BSA and Azide free (ab246001)

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Overview

  • Product name

    Anti-DAP12 antibody [EPR5173] - BSA and Azide free
    See all DAP12 primary antibodies

  • Description

    Rabbit monoclonal [EPR5173] to DAP12 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Fr, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human DAP12 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: O43914

  • General notes

    ab246001 is the carrier-free version of ab124834 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab246001 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 1.86 x 10 -10 M
    Learn more about KD

  • Storage buffer

    Constituent: PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5173

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab246001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P or IP.

    • Target

    • Function

      Non-covalently associates with activating receptors of the CD300 family. Cross-linking of CD300-TYROBP complexes results in cellular activation.

    • Tissue specificity

      Expressed at low levels in the early development of the hematopoietic system and in the promonocytic stage and at high levels in mature monocytes. Expressed in hematological cells and tissues such as peripheral blood leukocytes and spleen. Also found in bone marrow, lymph nodes, placenta, lung and liver. Expressed at lower levels in different parts of the brain especially in the basal ganglia and corpus callosum.

    • Involvement in disease

      Defects in TYROBP are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also called presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.

    • Sequence similarities

      Belongs to the TYROBP family.

    • Post-translational
      modifications

      Tyrosine phosphorylated.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • DAP 12 antibody
        • DAP12 antibody
        • DNAX activation protein 12 antibody
        • DNAX-activation protein 12 antibody
        • KAR-associated protein antibody
        • KARAP antibody
        • Killer activating receptor associated protein antibody
        • Killer-activating receptor-associated protein antibody
        • PLOSL antibody
        • TYOBP_HUMAN antibody
        • TYRO protein tyrosine kinase binding protein antibody
        • TYRO protein tyrosine kinase-binding protein antibody
        • TYROBP antibody
        see all

      References

      ab246001 has not yet been referenced specifically in any publications.

      Publishing research using ab246001? Please let us know so that we can cite the reference in this datasheet.

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