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    dap3-antibody-42c617-ab11928.pdf

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Epigenetics and Nuclear Signaling DNA / RNA Translation Ribosome
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Anti-DAP3 antibody [42C617] (ab11928)

  • Datasheet
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Western blot - Anti-DAP3 antibody [42C617] (ab11928)

    Key features and details

    • Mouse monoclonal [42C617] to DAP3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG1

    You may also be interested in

    Protein
    Product image
    Recombinant Human DAP3 protein (ab131869)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Primary
    Product image
    Anti-Collagen I antibody [EPR22894-89] (ab260043)

    View more associated products

    Overview

    • Product name

      Anti-DAP3 antibody [42C617]
      See all DAP3 primary antibodies
    • Description

      Mouse monoclonal [42C617] to DAP3
    • Host species

      Mouse
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      Synthetic peptide:corresponding to amino acids 45-52 and 358-371 of Human DAP3.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Jurkat whole-cell lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.02% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      42C617
    • Isotype

      IgG1
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • Translation
      • Ribosome
      • Cell Biology
      • Apoptosis
      • Mitochondrial
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial translation
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Mitochondrial markers
      • Metabolism
      • Pathways and Processes
      • Metabolism processes
      • Apoptosis
      • Cancer
      • Cell Death
      • Apoptosis
      • Mitochondrial
      • Cancer
      • Cell Death
      • Apoptosis
      • Metabolism

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Positive Controls

      • Jurkat whole cell lysate (ab7899)
    • Recombinant Protein

      • Recombinant Human DAP3 protein (ab131869)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab11928 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa.
    Notes
    WB
    Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa.

    Target

    • Function

      Involved in mediating interferon-gamma-induced cell death.
    • Tissue specificity

      Ubiquitous.
    • Cellular localization

      Mitochondrion.
    • Target information above from: UniProt accession P51398 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7818 Human
      • Omim: 602074 Human
      • SwissProt: P51398 Human
      • Unigene: 516746 Human
      • Alternative names

        • 28S ribosomal protein S29 antibody
        • 28S ribosomal protein S29 mitochondrial antibody
        • bMRP10 antibody
        • DAP 3 antibody
        • DAP-3 antibody
        • Dap3 antibody
        • Death associated protein 3 antibody
        • Death-associated protein 3 antibody
        • DKFZp686G12159 antibody
        • FLJ12817 antibody
        • Ionizing radiation resistance conferring protein antibody
        • MGC126058 antibody
        • MGC126059 antibody
        • Mitochondrial 28S ribosomal protein S29 antibody
        • mitochondrial antibody
        • MITOCHONDRIAL RIBOSOMAL PROTEIN S29 antibody
        • MRP S29 antibody
        • MRP-S29 antibody
        • MRPS29 antibody
        • OTTHUMP00000033480 antibody
        • OTTHUMP00000033481 antibody
        • PSD-95/SAP90-binding protein 3 antibody
        • RT29_HUMAN antibody
        • S29mt antibody
        • SAP90/PSD-95-associated protein 3 antibody
        • SAPAP3 antibody
        see all

      Images

      • Western blot - Anti-DAP3 antibody [42C617] (ab11928)
        Western blot - Anti-DAP3 antibody [42C617] (ab11928)
        Anti-DAP3 antibody [42C617] (ab11928) at 2 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

        Secondary
        Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Observed band size: 43 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (11)

      Publishing research using ab11928? Please let us know so that we can cite the reference in this datasheet.

      ab11928 has been referenced in 11 publications.

      • Reyes A  et al. RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases. PLoS Genet 16:e1008923 (2020). PubMed: 32735630
      • Van Haute L  et al. METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis. Nucleic Acids Res 47:10267-10281 (2019). PubMed: 31665743
      • Zaganelli S  et al. The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. J Biol Chem 292:4519-4532 (2017). PubMed: 28082677
      • Rozanska A  et al. The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome. Biochem J 474:2145-2158 (2017). WB . PubMed: 28512204
      • Rorbach J  et al. Human mitochondrial ribosomes can switch their structural RNA composition. Proc Natl Acad Sci U S A 113:12198-12201 (2016). WB . PubMed: 27729525
      View all Publications for this product

      Customer reviews and Q&As

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