Anti-DAP3 antibody [42C617] (ab11928)
![Western blot - Anti-DAP3 antibody [42C617] (ab11928)](https://www.abcam.com/ps/products/11/ab11928/Images/ab11928-19171-anti-dap3-antibody-42c617-western-blot.jpg "Western blot - Anti-DAP3 antibody [42C617] (ab11928)")
Key features and details
- Mouse monoclonal [42C617] to DAP3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-DAP3 antibody [42C617]
See all DAP3 primary antibodies -
Description
Mouse monoclonal [42C617] to DAP3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:corresponding to amino acids 45-52 and 358-371 of Human DAP3.
-
Positive control
- Jurkat whole-cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
42C617 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa.
|
| Notes |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa. |
Target
-
Function
Involved in mediating interferon-gamma-induced cell death. -
Tissue specificity
Ubiquitous. -
Cellular localization
Mitochondrion. - Information by UniProt
-
Database links
- Entrez Gene: 7818 Human
- Omim: 602074 Human
- SwissProt: P51398 Human
- Unigene: 516746 Human
-
Alternative names
- 28S ribosomal protein S29 antibody
- 28S ribosomal protein S29 mitochondrial antibody
- bMRP10 antibody
see all
Images
-
Western blot - Anti-DAP3 antibody [42C617] (ab11928)Anti-DAP3 antibody [42C617] (ab11928) at 2 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size: 43 kDa why is the actual band size different from the predicted?
Datasheets and documents
-
Datasheet download
References (11)
ab11928 has been referenced in 11 publications.
- Reyes A et al. RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases. PLoS Genet 16:e1008923 (2020). PubMed: 32735630
- Van Haute L et al. METTL15 introduces N4-methylcytidine into human mitochondrial 12S rRNA and is required for mitoribosome biogenesis. Nucleic Acids Res 47:10267-10281 (2019). PubMed: 31665743
- Zaganelli S et al. The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules. J Biol Chem 292:4519-4532 (2017). PubMed: 28082677
- Rozanska A et al. The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome. Biochem J 474:2145-2158 (2017). WB . PubMed: 28512204
- Rorbach J et al. Human mitochondrial ribosomes can switch their structural RNA composition. Proc Natl Acad Sci U S A 113:12198-12201 (2016). WB . PubMed: 27729525
