Key features and details
- Rabbit polyclonal to DAP3
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DAP3 antibody
See all DAP3 primary antibodies
DescriptionRabbit polyclonal to DAP3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Pig, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human DAP3 aa 250-300. The exact sequence is proprietary. (NP_004623.1).
Database link: P51398
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225622 was affinity purified using an epitope specific to DAP3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 46 kDa.|
FunctionInvolved in mediating interferon-gamma-induced cell death.
- Information by UniProt
- 28S ribosomal protein S29 antibody
- 28S ribosomal protein S29 mitochondrial antibody
- bMRP10 antibody
All lanes : Anti-DAP3 antibody (ab225622) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 46 kDa
Exposure time: 30 seconds
DAP3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225622 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225622 at 1 µg/ml.
Lane 1: ab225622 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab225622 has not yet been referenced specifically in any publications.