Overview

  • Product name

  • Description

    Rabbit polyclonal to DAP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Pig, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human DAP3 aa 250-300. The exact sequence is proprietary. (NP_004623.1).
    Database link: P51398

  • Positive control

    • WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab225622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 46 kDa.

Target

  • Function

    Involved in mediating interferon-gamma-induced cell death.
  • Tissue specificity

    Ubiquitous.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • 28S ribosomal protein S29 antibody
    • 28S ribosomal protein S29 mitochondrial antibody
    • bMRP10 antibody
    • DAP 3 antibody
    • DAP-3 antibody
    • Dap3 antibody
    • Death associated protein 3 antibody
    • Death-associated protein 3 antibody
    • DKFZp686G12159 antibody
    • FLJ12817 antibody
    • Ionizing radiation resistance conferring protein antibody
    • MGC126058 antibody
    • MGC126059 antibody
    • Mitochondrial 28S ribosomal protein S29 antibody
    • mitochondrial antibody
    • MITOCHONDRIAL RIBOSOMAL PROTEIN S29 antibody
    • MRP S29 antibody
    • MRP-S29 antibody
    • MRPS29 antibody
    • OTTHUMP00000033480 antibody
    • OTTHUMP00000033481 antibody
    • PSD-95/SAP90-binding protein 3 antibody
    • RT29_HUMAN antibody
    • S29mt antibody
    • SAP90/PSD-95-associated protein 3 antibody
    • SAPAP3 antibody
    see all

Images

  • All lanes : Anti-DAP3 antibody (ab225622) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 46 kDa


    Exposure time: 30 seconds
  • DAP3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225622 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225622 at 1 µg/ml.

    Lane 1: ab225622 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 seconds.

References

ab225622 has not yet been referenced specifically in any publications.

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