Product nameAnti-DAP3 antibody
See all DAP3 primary antibodies
DescriptionRabbit polyclonal to DAP3
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Pig, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human DAP3 aa 250-300. The exact sequence is proprietary. (NP_004623.1).
Database link: P51398
- WB: HeLa and HEK-293T whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab225622 was affinity purified using an epitope specific to DAP3 immobilized on solid support.
Our Abpromise guarantee covers the use of ab225622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 46 kDa.|
FunctionInvolved in mediating interferon-gamma-induced cell death.
- Information by UniProt
- 28S ribosomal protein S29 antibody
- 28S ribosomal protein S29 mitochondrial antibody
- bMRP10 antibody
All lanes : Anti-DAP3 antibody (ab225622) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 46 kDa
Exposure time: 30 seconds
DAP3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225622 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225622 at 1 µg/ml.
Lane 1: ab225622 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
ab225622 has not yet been referenced specifically in any publications.