Key features and details
- Rabbit polyclonal to DAP3
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DAP3 antibody
See all DAP3 primary antibodies
DescriptionRabbit polyclonal to DAP3
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human DAP3 (internal sequence). The exact sequence is proprietary.
Database link: P51398
- IHC-P: MDA-MB-231 xenograft tissue. WB: HepG2 whole cell lysate. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 46 kDa.|
|ICC/IF||1/100 - 1/1000.|
|IHC-P||1/100 - 1/1000.|
FunctionInvolved in mediating interferon-gamma-induced cell death.
- Information by UniProt
- 28S ribosomal protein S29 antibody
- 28S ribosomal protein S29 mitochondrial antibody
- bMRP10 antibody
Anti-DAP3 antibody (ab227257) at 1/1000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 46 kDa
10% SDS-PAGE gel.
Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for DAP3 (green) using ab227257 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).
Paraffin-embedded MDA-MB-231 xenograft tissue stained for DAP3 with ab227257 at 1/250 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227257 has not yet been referenced specifically in any publications.