Overview

  • Product name

  • Description

    Goat polyclonal to DAP3
  • Host species

    Goat
  • Specificity

    This antibody is expected to recognise both human isoforms of this protein.
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human DAP3 aa 387-398 (C terminal).
    Sequence:

    NPSLLERHCAYL


    (Peptide available as ab22922)

  • Positive control

    • WB: HeLa and HepG2 cell lysate and human kidney tissue lysate. ICC/IF: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab2637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 3 - 10 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 46 kDa.

Target

  • Function

    Involved in mediating interferon-gamma-induced cell death.
  • Tissue specificity

    Ubiquitous.
  • Cellular localization

    Mitochondrion.
  • Information by UniProt
  • Database links

  • Alternative names

    • 28S ribosomal protein S29 antibody
    • 28S ribosomal protein S29 mitochondrial antibody
    • bMRP10 antibody
    • DAP 3 antibody
    • DAP-3 antibody
    • Dap3 antibody
    • Death associated protein 3 antibody
    • Death-associated protein 3 antibody
    • DKFZp686G12159 antibody
    • FLJ12817 antibody
    • Ionizing radiation resistance conferring protein antibody
    • MGC126058 antibody
    • MGC126059 antibody
    • Mitochondrial 28S ribosomal protein S29 antibody
    • mitochondrial antibody
    • MITOCHONDRIAL RIBOSOMAL PROTEIN S29 antibody
    • MRP S29 antibody
    • MRP-S29 antibody
    • MRPS29 antibody
    • OTTHUMP00000033480 antibody
    • OTTHUMP00000033481 antibody
    • PSD-95/SAP90-binding protein 3 antibody
    • RT29_HUMAN antibody
    • S29mt antibody
    • SAP90/PSD-95-associated protein 3 antibody
    • SAPAP3 antibody
    see all

Images

  • Anti-DAP3 antibody (ab2637) at 0.3 µg/ml + Human kidney tissue lysate in RIPA buffer at 30 µg

    Predicted band size: 46 kDa



    Detected using chemiluminescence.

  • Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling DAP3 with ab2637 at 10 µg/ml. Cells were paraformaldehyde fixed and permeabilized with 0.15% triton. Primary antibody icubation was for 1 hour followed by incubation with an Alexa Fluor® 488-conjugated secondary antibody at 2 µg/ml. DAPI nuclear counterstain was used.

    Negative control: Unimmunized goat IgG (10 µg/ml) followed by an Alexa Fluor® 488-conjugated secondary antibody (2 µg/ml).

  • All lanes : Anti-DAP3 antibody (ab2637) at 0.3 µg/ml

    Lane 1 : HeLa cell lysate in RIPA buffer
    Lane 2 : HepG2 cell lysate in RIPA buffer

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 46 kDa



    Detected using chemiluminescence.

  • All lanes : Anti-DAP3 antibody (ab2637) at 1 µg/ml (for 1 hour)

    Lane 1 : HeLa cell lysate in RIPA buffer - control
    Lane 2 : HeLa cell lysate in RIPA buffer - after siRNA mediated DAP3 knock-down expression

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 46 kDa



    Level of knock-down relative to actin expression level determined by RT-PCR.

References

ab2637 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab2637.
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