Product nameDAPI Staining Solution
Sample typeAdherent cells, Suspension cells
DAPI Staining Solution (ab228549) is a fluorescent stain for labeling DNA in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells and fixed cells.
Molecular Weight: 350.25
DAPI’s absorption maximum when bound to double-stranded DNA is at 358 nm and its emission maximum is at 461 nm. Although is not as strongly fluorescent as when bound to DNA, it also binds to RNA, shifting its emission to around 500 nm.
DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like FITC and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 2 ml DAPI (10 mM solution in water) 1 x 2ml
This product has been referenced in:
- Li L et al. Mesenchymal stem cells with downregulated Hippo signaling attenuate lung injury in mice with lipopolysaccharide-induced acute respiratory distress syndrome. Int J Mol Med 43:1241-1252 (2019). Read more (PubMed: 30628652) »