Key features and details
- Rabbit monoclonal [EP1633Y] to DAPK2
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DAPK2 antibody [EP1633Y]
See all DAPK2 primary antibodies
DescriptionRabbit monoclonal [EP1633Y] to DAPK2
Specificityab51601 recognizes DAPK2.
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human DAPK2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9UIK4
- WB: Rat spleen lysate IHC-P: Human liver carcinoma. Flow Cyt: HeLa cells.
Previously labelled as DAP Kinase 2.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab51601 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCalcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
Tissue specificityUbiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- DAK2 antibody
- DAP kinase 2 antibody
- DAP kinase related protein 1 antibody
Anti-DAPK2 antibody [EP1633Y] (ab51601) at 1/2000 dilution + Rat spleen lysate at 10 µg
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Overlay histogram showing HeLa cells stained with ab51601 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51601, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunohistochemical staining of paraffin embedded human liver carcinoma using ab51601 (1/50). Secondary stained using Goat anti-rabbit HRP (1/2000).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab51601 has been referenced in 4 publications.
- Shiloh R et al. Non-canonical activation of DAPK2 by AMPK constitutes a new pathway linking metabolic stress to autophagy. Nat Commun 9:1759 (2018). PubMed: 29717115
- Chi HC et al. Thyroid hormone suppresses hepatocarcinogenesis via DAPK2 and SQSTM1-dependent selective autophagy. Autophagy 12:2271-2285 (2016). PubMed: 27653365
- Ber Y et al. DAPK2 is a novel regulator of mTORC1 activity and autophagy. Cell Death Differ 22:465-75 (2015). PubMed: 25361081
- Shoval Y et al. New Modularity of DAP-Kinases: Alternative Splicing of the DRP-1 Gene Produces a ZIPk-Like Isoform. PLoS One 6:e17344 (2011). WB ; Human . PubMed: 21408167