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Goat polyclonal to DAPP1
HumanPredicted to work with:
Peptide with sequence C-
, corresponding to C terminal amino acids 257-269 from the internal region (near C Terminus) of Human DAPP1 (NP_055210.2)
- Transfected HEK293 cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 32 kDa).
May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
Contains 1 PH domain.
Contains 1 SH2 domain.
Phosphorylated on tyrosine residues.
Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
Information by UniProt
- B cell adapter molecule of 32 kDa antibody
- B lymphocyte adapter protein Bam32 antibody
- B-cell adapter molecule of 32 kDa antibody
Western blot - Anti-DAPP1 antibody (ab123498)
Lane 1 :
Anti-DAPP1 antibody (ab123498) at 0.1 µg/mlLane 2 :
Anti-DAPP1 antibody (ab123498) at 1 mg/mlLane 3 :
Anti-DYKDDDDK Tag antibody at 1/3000 dilutionLanes 1 & 3 :
HEK293 lysate in RIPA buffer over expressing Human DAPP1 with DYKDDDDK tag Lane 2 :
Mock-transfected HEK293 Predicted band size:
32 kDaObserved band size:
35 kDa (why is the actual band size different from the predicted?
Primary incubations were for 1 hour. Detected by chemiluminescence.
has not yet been referenced specifically in any publications.
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