Overview

  • Product name

  • Description

    Rabbit polyclonal to DAPP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1 and 280 of Human DAPP1

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2 and Raji whole cell lysates; Gastric cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 32 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.

Target

  • Function

    May act as a B-cell-associated adapter that regulates B-cell antigen receptor (BCR)-signaling downstream of PI3K.
  • Tissue specificity

    Highly expressed in placenta and lung, followed by brain, heart, kidney, liver, pancreas and skeletal muscle. Expressed by B-lymphocytes, but not T-lymphocytes or nonhematopoietic cells.
  • Sequence similarities

    Contains 1 PH domain.
    Contains 1 SH2 domain.
  • Post-translational
    modifications

    Phosphorylated on tyrosine residues.
  • Cellular localization

    Cytoplasm. Membrane. Membrane-associated after cell stimulation leading to its translocation.
  • Information by UniProt
  • Database links

  • Alternative names

    • B cell adapter molecule of 32 kDa antibody
    • B lymphocyte adapter protein Bam32 antibody
    • B-cell adapter molecule of 32 kDa antibody
    • BAM32 antibody
    • DAPP1 antibody
    • DAPP1_HUMAN antibody
    • DKFZp667E0716 antibody
    • Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide antibody
    • Dual adaptor of phosphotyrosine and 3 phosphoinositides antibody
    • hDAPP1 antibody
    see all

Images

  • Anti-DAPP1 antibody (ab137374) at 1/500 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 32 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded gastric cancer tissue labelling DAPP1 using ab137374 at 1/100 dilution.

References

ab137374 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab137374.
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