Question (49189) | Anti-DARC antibody (ab40821)

Go to datasheet (ab40821)

Question

AbID: 40821, Anti-DARC antibody Rating: Poor Sample: Species: Human Type: Cell lysate - whole cell Loading amount: 50 µg Specification: human colorectal cancer cell line HCT116 Application: Application: Gel Running Conditions: Reduced Denaturing (12%) Blocking step: Milk as blocking agent for 1 hour at 25°C Blocking Concentration: 5% Other detail: Concentration: 0.2 µg/ml Incubation time: 16 hours at 4°C Diluent: 1X TBST Secondary Antibody: Name: Non-Abcam Antibody was used Conjugation: HRP Dilution: 1/5000 Detection: Detection method: ECL Exposure: 2 minutes and 0 seconds Positive Control: none Additional Data:

Answer

Thank you for your recent review of ab40821.
I would like to follow-up with you since the results with this antibody were so poor. Were there no bands at all on the blot, or were there any non-specific bands? Did you try any other concentrations of the antibody? What lysis buffer was used? Is the anti-goat secondary antibody working with other primary antibodies?
I look forward to hearing from you. We do fully guarantee this antibody to work in Western blot with human lysates, so I'd be happy to send a replacement or issue a credit or refund if you would like.
Please let me know if you have any questions or if there is anything else that we can do for you.

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