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AbID: 40821, Anti-DARC antibody Rating: Poor Sample: Species: Human Type: Cell lysate - whole cell Loading amount: 50 µg Specification: human colorectal cancer cell line HCT116 Application: Application: Gel Running Conditions: Reduced Denaturing (12%) Blocking step: Milk as blocking agent for 1 hour at 25°C Blocking Concentration: 5% Other detail: Concentration: 0.2 µg/ml Incubation time: 16 hours at 4°C Diluent: 1X TBST Secondary Antibody: Name: Non-Abcam Antibody was used Conjugation: HRP Dilution: 1/5000 Detection: Detection method: ECL Exposure: 2 minutes and 0 seconds Positive Control: none Additional Data:
Asked on May 31 2012
Thank you for your recent review of ab40821.
I would like to follow-up with you since the results with this antibody were so poor. Were there no bands at all on the blot, or were there any non-specific bands? Did you try any other concentrations of the antibody? What lysis buffer was used? Is the anti-goat secondary antibody working with other primary antibodies?
I look forward to hearing from you. We do fully guarantee this antibody to work in Western blot with human lysates, so I'd be happy to send a replacement or issue a credit or refund if you would like.
Please let me know if you have any questions or if there is anything else that we can do for you.
Answered on May 31 2012