Overview

  • Product name
    Anti-DARC antibody [EPR5205]
    See all DARC primary antibodies
  • Description
    Rabbit monoclonal [EPR5205] to DARC
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Fr, IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues within the intracellular domain of Human DARC (UniProt ID: Q16570)

  • Positive control
    • WB: Human fetal liver, Human fetal kidney, Human tonsil, kidney, stomach, TF 1 and K562 lysates; IHC-P: Human kidney tissue IP: TF-1 cells
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 25858253
IP 1/10 - 1/100.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

WB 1/1000 - 1/10000. Predicted molecular weight: 35 kDa.

Target

  • Function
    Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
  • Tissue specificity
    Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium.
  • Sequence similarities
    Belongs to the G-protein coupled receptor Duffy family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACKR1 antibody
    • Atypical chemokine receptor 1 antibody
    • CCBP1 antibody
    • CD234 antibody
    • DARC antibody
    • Dfy antibody
    • Duffy antigen/chemokine receptor antibody
    • duffy blood group antibody
    • Duffy blood group antigen antibody
    • Duffy blood group chemokine receptor antibody
    • DUFFY_HUMAN antibody
    • FY antibody
    • Fy glycoprotein antibody
    • Glycoprotein D antibody
    • GPD antibody
    • GpFy antibody
    • Plasmodium vivax receptor antibody
    • WBCQ1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling DARC with Purified ab137044 at 1:500 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • ab137044 (purified) at 1:30 dilution (2µg) immunoprecipitating DARC in TF-1 whole cell lysate.
    Lane 1 (input): TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10µg
    Lane 2 (+): ab137044 & TF-1 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137044 in TF-1 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/2000 dilution (Purified)

    Lane 1 : Human kidney lysates at 15 µg


    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution (Purified)

    Lane 1 : Human stomach lysates at 15 µg


    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling DARC with ab137044 at 1/100 dilution.

    This image was generated using the unpurified version of the product. 

  • All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal kidney lysate
    Lane 3 : Human tonsil lysate
    Lane 4 : TF 1 lysate
    Lane 5 : K562 lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 35 kDa



    This image was generated using the unpurified version of the product. 

References

This product has been referenced in:
  • Wan W  et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res 106:478-87 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25858253) »
  • Al-Alwan LA  et al. CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines. J Immunol 193:1416-26 (2014). Read more (PubMed: 24981451) »
See all 2 Publications for this product

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