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    darc-antibody-epr5205-ab137044.pdf

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RecombinantRabMAb

Recombinant Anti-DARC antibody [EPR5205] (ab137044)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)
  • Immunoprecipitation - Anti-DARC antibody [EPR5205] (ab137044)
  • Western blot - Anti-DARC antibody [EPR5205] (ab137044)
  • Western blot - Anti-DARC antibody [EPR5205] (ab137044)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)
  • Western blot - Anti-DARC antibody [EPR5205] (ab137044)
  • Anti-DARC antibody [EPR5205] (ab137044)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5205] to DARC
  • Suitable for: IP, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Biotin Carrier Free

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Assay
Product image
Aspartate Assay Kit (ab102512)
Protein
Product image
Recombinant Human DARC protein (ab114557)
Primary
Product image
Biotin Anti-DARC antibody [EPR5205] (ab199770)

View more associated products

Overview

  • Product name

    Anti-DARC antibody [EPR5205]
    See all DARC primary antibodies
  • Description

    Rabbit monoclonal [EPR5205] to DARC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver, Human fetal kidney, Human tonsil, kidney, stomach, TF 1 and K562 lysates; IHC-P: Human kidney tissue IP: TF-1 cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR5205
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • Cardiovascular
    • Blood
    • Other
    • Immunology
    • Innate Immunity
    • Chemokines
    • Alpha Chemokine Rec. (CXCR)
    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokine Rec. (CCR)
    • Microbiology
    • Organism
    • Eukaryotic Microorganism
    • Protozoa
    • Plasmodium
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR

Associated products

  • Alternative Versions

    • Biotin Anti-DARC antibody [EPR5205] (ab199770)
    • Anti-DARC antibody [EPR5205] - BSA and Azide free (ab240100)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human DARC protein (ab114557)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/10 - 1/100.
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
Notes
IP
1/10 - 1/100.
IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.

Target

  • Function

    Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
  • Tissue specificity

    Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium.
  • Sequence similarities

    Belongs to the G-protein coupled receptor Duffy family.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q16570 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2532 Human
    • Omim: 110700 Human
    • SwissProt: Q16570 Human
    • Unigene: 153381 Human
    • Alternative names

      • ACKR1 antibody
      • Atypical chemokine receptor 1 antibody
      • CCBP1 antibody
      • CD234 antibody
      • DARC antibody
      • Dfy antibody
      • Duffy antigen/chemokine receptor antibody
      • duffy blood group antibody
      • Duffy blood group antigen antibody
      • Duffy blood group chemokine receptor antibody
      • DUFFY_HUMAN antibody
      • FY antibody
      • Fy glycoprotein antibody
      • Glycoprotein D antibody
      • GPD antibody
      • GpFy antibody
      • Plasmodium vivax receptor antibody
      • WBCQ1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling DARC with Purified ab137044 at 1:500 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunoprecipitation - Anti-DARC antibody [EPR5205] (ab137044)
      Immunoprecipitation - Anti-DARC antibody [EPR5205] (ab137044)

      ab137044 (purified) at 1:30 dilution (2µg) immunoprecipitating DARC in TF-1 whole cell lysate.
      Lane 1 (input): TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10µg
      Lane 2 (+): ab137044 & TF-1 whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137044 in TF-1 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-DARC antibody [EPR5205] (ab137044)
      Western blot - Anti-DARC antibody [EPR5205] (ab137044)
      All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/2000 dilution (Purified)

      Lane 1 : Human kidney lysates at 15 µg


      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 35 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?

    • Western blot - Anti-DARC antibody [EPR5205] (ab137044)
      Western blot - Anti-DARC antibody [EPR5205] (ab137044)
      All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution (Purified)

      Lane 1 : Human stomach lysates at 15 µg


      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 35 kDa
      Observed band size: 40 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)

      Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling DARC with ab137044 at 1/100 dilution.

      This image was generated using the unpurified version of the product. 

      Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-DARC antibody [EPR5205] (ab137044)
      Western blot - Anti-DARC antibody [EPR5205] (ab137044)
      All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal kidney lysate
      Lane 3 : Human tonsil lysate
      Lane 4 : TF 1 lysate
      Lane 5 : K562 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 35 kDa



      This image was generated using the unpurified version of the product. 

    • Anti-DARC antibody [EPR5205] (ab137044)
      Anti-DARC antibody [EPR5205] (ab137044)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (2)

    Publishing research using ab137044? Please let us know so that we can cite the reference in this datasheet.

    ab137044 has been referenced in 2 publications.

    • Wan W  et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res 106:478-87 (2015). IHC-Fr ; Mouse . PubMed: 25858253
    • Al-Alwan LA  et al. CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines. J Immunol 193:1416-26 (2014). PubMed: 24981451

    Customer reviews and Q&As

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