Recombinant Anti-DARC antibody [EPR5205] (ab137044)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-DARC antibody [EPR5205]
See all DARC primary antibodies -
Description
Rabbit monoclonal [EPR5205] to DARC -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues within the intracellular domain of Human DARC (UniProt ID: Q16570)
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Positive control
- WB: Human fetal liver, Human fetal kidney, Human tonsil, kidney, stomach, TF 1 and K562 lysates; IHC-P: Human kidney tissue IP: TF-1 cells
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5205 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab137044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. PubMed: 25858253 | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
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| WB | 1/1000 - 1/10000. Predicted molecular weight: 35 kDa. |
Target
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Function
Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi. -
Tissue specificity
Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium. -
Sequence similarities
Belongs to the G-protein coupled receptor Duffy family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2532 Human
- Omim: 110700 Human
- SwissProt: Q16570 Human
- Unigene: 153381 Human
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Alternative names
- ACKR1 antibody
- Atypical chemokine receptor 1 antibody
- CCBP1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)](https://www.abcam.com/ps/products/137/ab137044/Images/ab137044-5-ab137044IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling DARC with Purified ab137044 at 1:500 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunoprecipitation - Anti-DARC antibody [EPR5205] (ab137044)](https://www.abcam.com/ps/products/137/ab137044/Images/ab137044-6-ab137044IP1.jpg)
Immunoprecipitation - Anti-DARC antibody [EPR5205] (ab137044)ab137044 (purified) at 1:30 dilution (2µg) immunoprecipitating DARC in TF-1 whole cell lysate.
Lane 1 (input): TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10µg
Lane 2 (+): ab137044 & TF-1 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137044 in TF-1 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. -
![Western blot - Anti-DARC antibody [EPR5205] (ab137044)](https://www.abcam.com/ps/products/137/ab137044/Images/ab137044-339720-ab137044WB2.jpg)
Western blot - Anti-DARC antibody [EPR5205] (ab137044)All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/2000 dilution (Purified)
Lane 1 : Human kidney lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-DARC antibody [EPR5205] (ab137044)](https://www.abcam.com/ps/products/137/ab137044/Images/ab137044-339719-ab137044WB1.jpg)
Western blot - Anti-DARC antibody [EPR5205] (ab137044)All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution (Purified)
Lane 1 : Human stomach lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)](https://www.abcam.com/ps/products/137/ab137044/Images/ab137044-151534-anti-darc-antibody-epr5205-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling DARC with ab137044 at 1/100 dilution.
This image was generated using the unpurified version of the product.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-DARC antibody [EPR5205] (ab137044)](https://www.abcam.com/ps/products/137/ab137044/Images/ab137044-151535-anti-darc-antibody-epr5205-western-blot.jpg)
Western blot - Anti-DARC antibody [EPR5205] (ab137044)All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human tonsil lysate
Lane 4 : TF 1 lysate
Lane 5 : K562 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 35 kDaThis image was generated using the unpurified version of the product.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wan W et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res 106:478-87 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25858253) »
- Al-Alwan LA et al. CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines. J Immunol 193:1416-26 (2014). Read more (PubMed: 24981451) »
