Name: Anti-DARC antibody [EPR5205]
Brand: RabMAb
SKU: ab137044

Product name

Anti-DARC antibody [EPR5205]
See all DARC primary antibodies
Description

Rabbit monoclonal [EPR5205] to DARC
Host species

Rabbit
Tested applications

Suitable for: IHC-Fr, IP, IHC-P, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide corresponding to residues within the intracellular domain of Human DARC (UniProt ID: Q16570)
Positive control
- WB: Human fetal liver, Human fetal kidney, Human tonsil, kidney, stomach, TF 1 and K562 lysates; IHC-P: Human kidney tissue IP: TF-1 cells
General notes

Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR5205
Isotype

IgG
Research areas

Alternative Versions
- Anti-DARC antibody [EPR5205] (Biotin) (ab199770)
- Anti-DARC antibody [EPR5205] - BSA and Azide free (ab240100)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit (ab102918)
- APC Conjugation Kit (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human DARC protein (ab114557)

Our Abpromise guarantee covers the use of ab137044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-Fr		Use at an assay dependent concentration. PubMed: 25858253
IP		1/10 - 1/100.
IHC-P		1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250.
WB		1/1000 - 1/10000. Predicted molecular weight: 35 kDa.

Function

Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
Tissue specificity

Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium.
Sequence similarities

Belongs to the G-protein coupled receptor Duffy family.
Cellular localization

Membrane.
Target information above from: UniProt accession Q16570 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2532 Human
- Omim: 110700 Human
- SwissProt: Q16570 Human
- Unigene: 153381 Human
Alternative names
- ACKR1 antibody
- Atypical chemokine receptor 1 antibody
- CCBP1 antibody
- CD234 antibody
- DARC antibody
- Dfy antibody
- Duffy antigen/chemokine receptor antibody
- duffy blood group antibody
- Duffy blood group antigen antibody
- Duffy blood group chemokine receptor antibody
- DUFFY_HUMAN antibody
- FY antibody
- Fy glycoprotein antibody
- Glycoprotein D antibody
- GPD antibody
- GpFy antibody
- Plasmodium vivax receptor antibody
- WBCQ1 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling DARC with Purified ab137044 at 1:500 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunoprecipitation - Anti-DARC antibody [EPR5205] (ab137044)

ab137044 (purified) at 1:30 dilution (2µg) immunoprecipitating DARC in TF-1 whole cell lysate.
Lane 1 (input): TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10µg
Lane 2 (+): ab137044 & TF-1 whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137044 in TF-1 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-DARC antibody [EPR5205] (ab137044)

All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/2000 dilution (Purified)

Lane 1 : Human kidney lysates at 15 µg

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 35 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?
Western blot - Anti-DARC antibody [EPR5205] (ab137044)

All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution (Purified)

Lane 1 : Human stomach lysates at 15 µg

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR5205] (ab137044)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling DARC with ab137044 at 1/100 dilution.

This image was generated using the unpurified version of the product.
Western blot - Anti-DARC antibody [EPR5205] (ab137044)

All lanes : Anti-DARC antibody [EPR5205] (ab137044) at 1/1000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lane 3 : Human tonsil lysate
Lane 4 : TF 1 lysate
Lane 5 : K562 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP conjugated Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 35 kDa

This image was generated using the unpurified version of the product.

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Wan W et al. Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice. Cardiovasc Res 106:478-87 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25858253) »
Al-Alwan LA et al. CXCL1 inhibits airway smooth muscle cell migration through the decoy receptor Duffy antigen receptor for chemokines. J Immunol 193:1416-26 (2014). Read more (PubMed: 24981451) »

See all 2 Publications for this product

Publishing research using ab137044? Please let us know so that we can cite the reference in this datasheet.

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Recombinant Anti-DARC antibody [EPR5205] (ab137044)

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