Recombinant
RabMAb

Recombinant Anti-DARC antibody [EPR5205] - BSA and Azide free (ab240100)

Overview

  • Product name

    Anti-DARC antibody [EPR5205] - BSA and Azide free
    See all DARC primary antibodies
  • Description

    Rabbit monoclonal [EPR5205] to DARC - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DARC aa 300-400 (intracellular). The exact sequence is proprietary.

  • Positive control

    • IP: TF-1 cells IHC-P: Human kidney tissue
  • General notes

    ab240100 is the carrier-free version of ab137044 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240100 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 25858253
WB Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.

Target

  • Function

    Non-specific receptor for many chemokines such as IL-8, GRO, RANTES, MCP-1 and TARC. It is also the receptor for the human malaria parasites Plasmodium vivax and Plasmodium knowlesi.
  • Tissue specificity

    Found in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along postcapillary venules throughout the body, except in the adult liver. Erythroid cells and postcapillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium.
  • Sequence similarities

    Belongs to the G-protein coupled receptor Duffy family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ACKR1 antibody
    • Atypical chemokine receptor 1 antibody
    • CCBP1 antibody
    • CD234 antibody
    • DARC antibody
    • Dfy antibody
    • Duffy antigen/chemokine receptor antibody
    • duffy blood group antibody
    • Duffy blood group antigen antibody
    • Duffy blood group chemokine receptor antibody
    • DUFFY_HUMAN antibody
    • FY antibody
    • Fy glycoprotein antibody
    • Glycoprotein D antibody
    • GPD antibody
    • GpFy antibody
    • Plasmodium vivax receptor antibody
    • WBCQ1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling DARC with Purified ab137044 at 1:500 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • ab137044 (purified) at 1:30 dilution (2µg) immunoprecipitating DARC in TF-1 whole cell lysate.
    Lane 1 (input): TF-1 (Human Erythroleukemia erythroblast) whole cell lysate 10µg
    Lane 2 (+): ab137044 & TF-1 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab137044 in TF-1 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137044).

     

  • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling DARC with ab137044 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137044).

    This image was generated using the unpurified version of the product. 

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

References

ab240100 has not yet been referenced specifically in any publications.

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