Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP721Y] to DARPP32
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-DARPP32 antibody [EP721Y]
See all DARPP32 primary antibodies
DescriptionRabbit monoclonal [EP721Y] to DARPP32
Tested applicationsSuitable for: ICC/IF, WB, IHC-P, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within DARPP32 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
- Human brain lysate and human gastric adenocarcinoma. SH-SY5Y (human neuroblastoma) and human fetal forebrain tissue lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.11% BSA, 59% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab40802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|WB||1/5000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Unpurified format: 1/5,000 - 1/20,000
|IHC-P||1/50 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionInhibitor of protein-phosphatase 1.
Sequence similaritiesBelongs to the protein phosphatase inhibitor 1 family.
modificationsDopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.
- Information by UniProt
- DARPP 32 antibody
- DARPP-32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
Anti-DARPP32 antibody [EP721Y] (ab40802) at 1/50000 dilution + Human brain tissue lysates. at 10 µg
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 32 kDa
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands.
ab40802 staining DARPP32 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.
Negative control 1: PBS in place of primary antibody.
ab40802 staining DARPP32 in SH-SY5Y (human neuroblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab40802 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Immunohistochemical analysis human gastric adenocarcinoma using ab40802. (dilution 1:250)
ab40802 immunoprecipitating DARPP32. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/50 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.
Lane 1: Human fetal forebrain whole cell lysate (10ug)
Lane 2: Human fetal forebrain whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab40802 in Human fetal forebrain whole cell lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab40802 has not yet been referenced specifically in any publications.