Recombinant Anti-DARPP32 antibody [EP721Y] (ab40802)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP721Y] to DARPP32
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DARPP32 antibody [EP721Y]
See all DARPP32 primary antibodies -
Description
Rabbit monoclonal [EP721Y] to DARPP32 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human brain lysate and human gastric adenocarcinoma. SH-SY5Y (human neuroblastoma) and human fetal forebrain tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.11% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP721Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/100.
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WB |
1/5000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Unpurified format: 1/5,000 - 1/20,000 |
|
IHC-P |
1/50 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IP |
1/50.
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Notes |
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ICC/IF
1/50 - 1/100. |
WB
1/5000 - 1/50000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). Unpurified format: 1/5,000 - 1/20,000 |
IHC-P
1/50 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
1/50. |
Target
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Function
Inhibitor of protein-phosphatase 1. -
Sequence similarities
Belongs to the protein phosphatase inhibitor 1 family. -
Post-translational
modificationsDopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 84152 Human
- Omim: 604399 Human
- SwissProt: Q9UD71 Human
- Unigene: 286192 Human
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Alternative names
- DARPP 32 antibody
- DARPP-32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
see all
Images
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Anti-DARPP32 antibody [EP721Y] (ab40802) at 1/50000 dilution + Human brain tissue lysates. at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 32 kDa
Additional bands at: 32 kDa. We are unsure as to the identity of these extra bands. -
ab40802 staining DARPP32 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody.
Negative control 1: PBS in place of primary antibody.
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ab40802 staining DARPP32 in SH-SY5Y (human neuroblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 and ab150120 were used as counterstains for primary antibody ab40802 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) -
Immunohistochemical analysis human gastric adenocarcinoma using ab40802. (dilution 1:250)
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ab40802 immunoprecipitating DARPP32. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/50 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.
Lane 1: Human fetal forebrain whole cell lysate (10ug)
Lane 2: Human fetal forebrain whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab40802 in Human fetal forebrain whole cell lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab40802 has been referenced in 3 publications.
- Sanders B et al. Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants. Nat Commun 13:27 (2022). PubMed: 35031607
- Lange J et al. Mislocalization of Nucleocytoplasmic Transport Proteins in Human Huntington's Disease PSC-Derived Striatal Neurons. Front Cell Neurosci 15:742763 (2021). PubMed: 34658796
- André EM et al. A Combinatorial Cell and Drug Delivery Strategy for Huntington's Disease Using Pharmacologically Active Microcarriers and RNAi Neuronally-Committed Mesenchymal Stromal Cells. Pharmaceutics 11:N/A (2019). PubMed: 31614758