Overview

  • Product name
    Anti-DARPP32 (phospho T75) antibody
    See all DARPP32 primary antibodies
  • Description
    Rabbit polyclonal to DARPP32 (phospho T75)
  • Host species
    Rabbit
  • Specificity
    This antibody detects endogenous levels of DARPP32 only when phosphorylated at threonine 75.
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human DARPP32 around the phosphorylation site of threonine 75 (A-Y-phosphoT-P-P).

  • Positive control
    • Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg+2 and Ca+2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    The antibody was purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab51114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/40000.
WB 1/3000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Inhibitor of protein-phosphatase 1.
  • Sequence similarities
    Belongs to the protein phosphatase inhibitor 1 family.
  • Post-translational
    modifications
    Dopamine- and cyclic AMP-regulated neuronal phosphoprotein.
    Phosphorylation of Thr-34 is required for activity.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DARPP 32 antibody
    • DARPP-32 antibody
    • Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
    • Dopamine and cAMP regulated neuronal phosphoprotein antibody
    • Dopamine and cAMP regulated phosphoprotein antibody
    • Dopamine and cAMP regulated phosphoprotein DARPP 32 antibody
    • Dopamine and cAMP regulated phosphoprotein DARPP32 antibody
    • Dopamine- and cAMP-regulated neuronal phosphoprotein antibody
    • FLJ20940 antibody
    • IPPD antibody
    • Neuronal phosphoprotein DARPP 32 antibody
    • PPP1R1B antibody
    • PPR1B_HUMAN antibody
    • Protein phosphatase 1 regulatory (inhibitor) subunit 1B antibody
    • Protein phosphatase 1 regulatory inhibitor subunit 1B antibody
    • Protein phosphatase 1 regulatory subunit 1B antibody
    see all

Images

  • Ab51114 staining human DARPP32 in human brain tissue by immunohistochemistry using paraffin embedded tissue.
  • All lanes : Anti-DARPP32 (phospho T75) antibody (ab51114) at 1 µg/ml

    Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 32 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:
  • Yoon Y  et al. Genetic Ablation of EWS RNA Binding Protein 1 (EWSR1) Leads to Neuroanatomical Changes and Motor Dysfunction in Mice. Exp Neurobiol 27:103-111 (2018). Read more (PubMed: 29731676) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab51114.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up