Product nameAnti-DARPP32 (phospho T75) antibody
See all DARPP32 primary antibodies
DescriptionRabbit polyclonal to DARPP32 (phospho T75)
SpecificityThis antibody detects endogenous levels of DARPP32 only when phosphorylated at threonine 75.
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic phosphopeptide derived from human DARPP32 around the phosphorylation site of threonine 75 (A-Y-phosphoT-P-P).
- Human brain tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab51114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/3000. Detects a band of approximately 38 kDa (predicted molecular weight: 32 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionInhibitor of protein-phosphatase 1.
Sequence similaritiesBelongs to the protein phosphatase inhibitor 1 family.
modificationsDopamine- and cyclic AMP-regulated neuronal phosphoprotein.
Phosphorylation of Thr-34 is required for activity.
- Information by UniProt
- DARPP 32 antibody
- DARPP-32 antibody
- Dopamine and cAMP regulated neuronal phosphoprotein 32 antibody
Ab51114 staining human DARPP32 in human brain tissue by immunohistochemistry using paraffin embedded tissue.
All lanes : Anti-DARPP32 (phospho T75) antibody (ab51114) at 1 µg/ml
Lane 1 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 32 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?