Anti-DARS antibody (ab151974)
Key features and details
- Rabbit polyclonal to DARS
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DARS antibody
See all DARS primary antibodies -
Description
Rabbit polyclonal to DARS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment corresponding to a region within amino acids 1-308 of Human DARS (NP_001340).
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Positive control
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysate; A431 cells; SW480 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 57 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 57 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. -
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 510162 Cow
- Entrez Gene: 1615 Human
- Entrez Gene: 226414 Mouse
- Entrez Gene: 116483 Rat
- Omim: 603084 Human
- SwissProt: Q3SYZ4 Cow
- SwissProt: P14868 Human
- SwissProt: Q922B2 Mouse
see all -
Alternative names
- Aspartate tRNA ligase 1 cytoplasmic antibody
- Aspartate tRNA ligase antibody
- Aspartate--tRNA ligase antibody
see all
Images
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Western blot - Anti-DARS antibody (ab151974)All lanes : Anti-DARS antibody (ab151974) at 1/1000 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lane 3 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-DARS antibody (ab151974)Immunofluorescence analysis of methanol- fixed A431 cells, labeling DARS with ab151974 a 1/200 dilution. Lower image costained with Hoerchst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARS antibody (ab151974)Immunohistochemical analysis of paraffin embedded Human SW480 xenograft tissue labeling DARS with ab151974 at a 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab151974 has been referenced in 1 publication.
- Jiao M et al. DARS-AS1 promotes clear cell renal cell carcinoma by sequestering miR-194-5p to up-regulate DARS. Biomed Pharmacother 128:110323 (2020). PubMed: 32526457