Overview

  • Product name

  • Description

    Rabbit polyclonal to DARS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 1-308 of Human DARS (NP_001340).

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysate; A431 cells; SW480 xenograft tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-DARS antibody (ab151974) at 1/1000 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate
    Lane 3 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 57 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol- fixed A431 cells, labeling DARS with ab151974 a 1/200 dilution. Lower image costained with Hoerchst 33342.
  • Immunohistochemical analysis of paraffin embedded Human SW480 xenograft tissue labeling DARS with ab151974 at a 1/500 dilution.

References

ab151974 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151974.
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