Recombinant Anti-DARS antibody [EPR14847(B)] (ab182157)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14847(B)] to DARS
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-DARS antibody [EPR14847(B)]
See all DARS primary antibodies -
Description
Rabbit monoclonal [EPR14847(B)] to DARS -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human DARS aa 400-500. The exact sequence is proprietary.
Database link: P14868 -
Positive control
- Jurkat, HeLa, Raji and K562 cell lysate. K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14847(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab182157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). | |
| Flow Cyt | 1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
Target
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Function
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. -
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1615 Human
- Entrez Gene: 226414 Mouse
- Entrez Gene: 116483 Rat
- Omim: 603084 Human
- SwissProt: P14868 Human
- SwissProt: Q922B2 Mouse
- SwissProt: P15178 Rat
- Unigene: 503787 Human
see all -
Alternative names
- Aspartate tRNA ligase 1 cytoplasmic antibody
- Aspartate tRNA ligase antibody
- Aspartate--tRNA ligase antibody
see all
Images
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![Western blot - Anti-DARS antibody [EPR14847(B)] (ab182157)](https://www.abcam.com/ps/products/182/ab182157/Images/ab182157-213910-anti-dars-antibody-epr14847b-western-blot.jpg)
Western blot - Anti-DARS antibody [EPR14847(B)] (ab182157)All lanes : Anti-DARS antibody [EPR14847(B)] (ab182157) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
![Flow Cytometry - Anti-DARS antibody [EPR14847(B)] (ab182157)](https://www.abcam.com/ps/products/182/ab182157/Images/ab182157-213909-anti-dars-antibody-epr14847b-flow-cytometry.jpg)
Flow Cytometry - Anti-DARS antibody [EPR14847(B)] (ab182157)Flow cytometry analysis of DARS expression in K562 cells using ab182157 at 1/40 dilution (red) and a rabbit IgG as negative control (green).
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![Anti-DARS antibody [EPR14847(B)] (ab182157)](https://www.abcam.com/ps/products/182/ab182157/Images/ab182157-3-benefits-of-recombinant-antibodies.png)
Anti-DARS antibody [EPR14847(B)] (ab182157)
Datasheets and documents
Certificate of Compliance
References (1)
ab182157 has been referenced in 1 publication.
- Luisier R et al. Intron retention and nuclear loss of SFPQ are molecular hallmarks of ALS. Nat Commun 9:2010 (2018). PubMed: 29789581
