RecombinantRabMAb

Recombinant Anti-DARS antibody [EPR14847(B)] (ab182157)

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Western blot - Anti-DARS antibody [EPR14847(B)] (ab182157)
  • Flow Cytometry (Intracellular) - Anti-DARS antibody [EPR14847(B)] (ab182157)
  • Anti-DARS antibody [EPR14847(B)] (ab182157)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14847(B)] to DARS
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-DARS antibody [EPR14847(B)]
    See all DARS primary antibodies

  • Description

    Rabbit monoclonal [EPR14847(B)] to DARS

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, HeLa, Raji and K562 cell lysate. K562 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14847(B)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Notes
Flow Cyt (Intra)
1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.

  • Sequence similarities

    Belongs to the class-II aminoacyl-tRNA synthetase family.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Aspartate tRNA ligase 1 cytoplasmic antibody
    • Aspartate tRNA ligase antibody
    • Aspartate--tRNA ligase antibody
    • Aspartyl tRNA synthetase antibody
    • Aspartyl tRNA synthetase cytoplasmic antibody
    • Aspartyl-tRNA synthetase antibody
    • AspRS antibody
    • Cell proliferation inducing gene 40 protein antibody
    • Cell proliferation inducing protein 40 antibody
    • Cell proliferation-inducing gene 40 protein antibody
    • cytoplasmic antibody
    • Dars antibody
    • DKFZp781B11202 antibody
    • MGC111579 antibody
    • SYDC_HUMAN antibody
    see all

Images

  • Western blot - Anti-DARS antibody [EPR14847(B)] (ab182157)
    Western blot - Anti-DARS antibody [EPR14847(B)] (ab182157)
    All lanes : Anti-DARS antibody [EPR14847(B)] (ab182157) at 1/5000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

  • Flow Cytometry (Intracellular) - Anti-DARS antibody [EPR14847(B)] (ab182157)
    Flow Cytometry (Intracellular) - Anti-DARS antibody [EPR14847(B)] (ab182157)

    Intracellular Flow Cytometry analysis of DARS expression in K562 cells using ab182157 at 1/40 dilution (red) and a rabbit IgG as negative control (green). 

     

     

  • Anti-DARS antibody [EPR14847(B)] (ab182157)
    Anti-DARS antibody [EPR14847(B)] (ab182157)

References (1)

Publishing research using ab182157? Please let us know so that we can cite the reference in this datasheet.

ab182157 has been referenced in 1 publication.

  • Luisier R  et al. Intron retention and nuclear loss of SFPQ are molecular hallmarks of ALS. Nat Commun 9:2010 (2018). PubMed: 29789581

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