Recombinant Anti-DARS antibody [EPR14847(B)] (ab182157)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-DARS antibody [EPR14847(B)]
See all DARS primary antibodies -
Description
Rabbit monoclonal [EPR14847(B)] to DARS -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human DARS aa 400-500. The exact sequence is proprietary.
Database link: P14868 -
Positive control
- Jurkat, HeLa, Raji and K562 cell lysate. K562 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14847(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab182157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). | |
| Flow Cyt | 1/40. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. -
Sequence similarities
Belongs to the class-II aminoacyl-tRNA synthetase family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1615 Human
- Entrez Gene: 226414 Mouse
- Entrez Gene: 116483 Rat
- Omim: 603084 Human
- SwissProt: P14868 Human
- SwissProt: Q922B2 Mouse
- SwissProt: P15178 Rat
- Unigene: 503787 Human
see all -
Alternative names
- Aspartate tRNA ligase 1 cytoplasmic antibody
- Aspartate tRNA ligase antibody
- Aspartate--tRNA ligase antibody
see all
Images
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![Western blot - Anti-DARS antibody [EPR14847(B)] (ab182157)](https://www.abcam.com/ps/products/182/ab182157/Images/ab182157-213910-anti-dars-antibody-epr14847b-western-blot.jpg)
Western blot - Anti-DARS antibody [EPR14847(B)] (ab182157)All lanes : Anti-DARS antibody [EPR14847(B)] (ab182157) at 1/5000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Raji cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 57 kDa
Observed band size: 57 kDa -
![Flow Cytometry - Anti-DARS antibody [EPR14847(B)] (ab182157)](https://www.abcam.com/ps/products/182/ab182157/Images/ab182157-213909-anti-dars-antibody-epr14847b-flow-cytometry.jpg)
Flow Cytometry - Anti-DARS antibody [EPR14847(B)] (ab182157)Flow cytometry analysis of DARS expression in K562 cells using ab182157 at 1/40 dilution (red) and a rabbit IgG as negative control (green).
Datasheets and documents
References
This product has been referenced in:
- Luisier R et al. Intron retention and nuclear loss of SFPQ are molecular hallmarks of ALS. Nat Commun 9:2010 (2018). Read more (PubMed: 29789581) »
