Overview

  • Product name

    Anti-DARS antibody [EPR14847(B)]
    See all DARS primary antibodies
  • Description

    Rabbit monoclonal [EPR14847(B)] to DARS
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DARS aa 400-500. The exact sequence is proprietary.
    Database link: P14868

  • Positive control

    • Jurkat, HeLa, Raji and K562 cell lysate. K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14847(B)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.
  • Sequence similarities

    Belongs to the class-II aminoacyl-tRNA synthetase family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Aspartate tRNA ligase 1 cytoplasmic antibody
    • Aspartate tRNA ligase antibody
    • Aspartate--tRNA ligase antibody
    • Aspartyl tRNA synthetase antibody
    • Aspartyl tRNA synthetase cytoplasmic antibody
    • Aspartyl-tRNA synthetase antibody
    • AspRS antibody
    • Cell proliferation inducing gene 40 protein antibody
    • Cell proliferation inducing protein 40 antibody
    • Cell proliferation-inducing gene 40 protein antibody
    • cytoplasmic antibody
    • Dars antibody
    • DKFZp781B11202 antibody
    • MGC111579 antibody
    • SYDC_HUMAN antibody
    see all

Images

  • All lanes : Anti-DARS antibody [EPR14847(B)] (ab182157) at 1/5000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

  • Flow cytometry analysis of DARS expression in K562 cells using ab182157 at 1/40 dilution (red) and a rabbit IgG as negative control (green).

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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