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    dars2-antibody-ab154606.pdf

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Signal Transduction Metabolism Mitochondrial
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Anti-DARS2 antibody (ab154606)

  • Datasheet
  • SDS
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Western blot - Anti-DARS2 antibody (ab154606)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARS2 antibody (ab154606)

Key features and details

  • Rabbit polyclonal to DARS2
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-DARS2 antibody
    See all DARS2 primary antibodies
  • Description

    Rabbit polyclonal to DARS2
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 9-274 of Human DARS2 (Uniprot ID: Q6PI48).

  • Positive control

    • NT2D1, IMR32 and MCF7 cell lysates; Human colon carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • tRNA synthetase
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial translation
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • MCF7 whole cell lysate (ab29537)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab154606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Cow
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Relevance

    Disease: Leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family.
  • Cellular localization

    Mitochondrion matrix.
  • Database links

    • Entrez Gene: 538772 Cow
    • Entrez Gene: 55157 Human
    • Entrez Gene: 226539 Mouse
    • Entrez Gene: 304919 Rat
    • Omim: 610956 Human
    • SwissProt: A6QPU5 Cow
    • SwissProt: Q6PI48 Human
    • SwissProt: Q8BIP0 Mouse
    • SwissProt: Q3KRD0 Rat
    • Unigene: 647707 Human
    • Unigene: 28125 Mouse
    • Unigene: 137838 Rat
    see all
  • Alternative names

    • Aspartate tRNA ligase 2 mitochondrial antibody
    • Aspartate tRNA ligase antibody
    • Aspartate tRNA ligase mitochondrial antibody
    • Aspartate--tRNA ligase antibody
    • Aspartyl tRNA synthetase 2 (mitochondrial) antibody
    • Aspartyl tRNA synthetase mitochondrial antibody
    • Aspartyl-tRNA synthetase antibody
    • AspRS antibody
    • DARS2 antibody
    • FLJ10514 antibody
    • LBSL antibody
    • mitochondrial antibody
    • MT ASPRS antibody
    • RP3 383J4.2 antibody
    • SYDM_HUMAN antibody
    see all

Images

  • Western blot - Anti-DARS2 antibody (ab154606)
    Western blot - Anti-DARS2 antibody (ab154606)
    Anti-DARS2 antibody (ab154606) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size: 74 kDa



    7.5% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARS2 antibody (ab154606)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARS2 antibody (ab154606)
    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling DARS2 with ab154606 at 1/250 dilution.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1)

    Publishing research using ab154606? Please let us know so that we can cite the reference in this datasheet.

    ab154606 has been referenced in 1 publication.

    • Qin X  et al. Upregulation of DARS2 by HBV promotes hepatocarcinogenesis through the miR-30e-5p/MAPK/NFAT5 pathway. J Exp Clin Cancer Res 36:148 (2017). PubMed: 29052520

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