Anti-DARS2 antibody (ab154606)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-DARS2 antibody
See all DARS2 primary antibodies -
Description
Rabbit polyclonal to DARS2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow
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Immunogen
Recombinant fragment corresponding to a region within amino acids 9-274 of Human DARS2 (Uniprot ID: Q6PI48).
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Positive control
- NT2D1, IMR32 and MCF7 cell lysates; Human colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab154606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 74 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
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Relevance
Disease: Leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. -
Cellular localization
Mitochondrion matrix. -
Database links
- Entrez Gene: 538772 Cow
- Entrez Gene: 55157 Human
- Entrez Gene: 226539 Mouse
- Entrez Gene: 304919 Rat
- Omim: 610956 Human
- SwissProt: A6QPU5 Cow
- SwissProt: Q6PI48 Human
- SwissProt: Q8BIP0 Mouse
see all -
Alternative names
- Aspartate tRNA ligase 2 mitochondrial antibody
- Aspartate tRNA ligase antibody
- Aspartate tRNA ligase mitochondrial antibody
see all
Images
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Western blot - Anti-DARS2 antibody (ab154606)Anti-DARS2 antibody (ab154606) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 74 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARS2 antibody (ab154606)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling DARS2 with ab154606 at 1/250 dilution.
References
This product has been referenced in:
- Qin X et al. Upregulation of DARS2 by HBV promotes hepatocarcinogenesis through the miR-30e-5p/MAPK/NFAT5 pathway. J Exp Clin Cancer Res 36:148 (2017). Read more (PubMed: 29052520) »
