Anti-DARS2 antibody (ab154606)
Key features and details
- Rabbit polyclonal to DARS2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DARS2 antibody
See all DARS2 primary antibodies -
Description
Rabbit polyclonal to DARS2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment corresponding to a region within amino acids 9-274 of Human DARS2 (Uniprot ID: Q6PI48).
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Positive control
- NT2D1, IMR32 and MCF7 cell lysates; Human colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Cow
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
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Relevance
Disease: Leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. -
Cellular localization
Mitochondrion matrix. -
Database links
- Entrez Gene: 538772 Cow
- Entrez Gene: 55157 Human
- Entrez Gene: 226539 Mouse
- Entrez Gene: 304919 Rat
- Omim: 610956 Human
- SwissProt: A6QPU5 Cow
- SwissProt: Q6PI48 Human
- SwissProt: Q8BIP0 Mouse
see all -
Alternative names
- Aspartate tRNA ligase 2 mitochondrial antibody
- Aspartate tRNA ligase antibody
- Aspartate tRNA ligase mitochondrial antibody
see all
Images
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Western blot - Anti-DARS2 antibody (ab154606)Anti-DARS2 antibody (ab154606) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 74 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARS2 antibody (ab154606)Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue labeling DARS2 with ab154606 at 1/250 dilution.
Protocols
References (1)
ab154606 has been referenced in 1 publication.
- Qin X et al. Upregulation of DARS2 by HBV promotes hepatocarcinogenesis through the miR-30e-5p/MAPK/NFAT5 pathway. J Exp Clin Cancer Res 36:148 (2017). PubMed: 29052520
