For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    daxx-antibody-ab2017.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Apoptosis Extracellular Signals Death Ligands
Share by email

Anti-Daxx antibody (ab2017)

  • Datasheet
Submit a review Q&A (3)References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-Daxx antibody (ab2017)

    Key features and details

    • Rabbit polyclonal to Daxx
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Daxx protein (ab131785)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Knockout
    Product image
    Human DAXX knockout HeLa cell line (ab265233)

    View more associated products

    Overview

    • Product name

      Anti-Daxx antibody
      See all Daxx primary antibodies
    • Description

      Rabbit polyclonal to Daxx
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      ICC/IF
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human Daxx aa 722-740.
      Sequence:

      TSVATQCDPEEIIVLSDSD


      (Peptide available as ab7879)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.2
      Preservative: 0.02% Sodium azide
    • Concentration information loading...
    • Purity

      DEAE-Chromatography
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cell Biology
      • Apoptosis
      • Extracellular Signals
      • Death Ligands
      • Microbiology
      • Interspecies Interaction
      • Host Virus Interaction
      • Epigenetics and Nuclear Signaling
      • Chromatin Remodeling
      • Histone chaperones

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Daxx protein (ab131785)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2017 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Human
    Application Abreviews Notes
    ICC/IF
    Use a concentration of 10 µg/ml.
    Notes
    ICC/IF
    Use a concentration of 10 µg/ml.

    Target

    • Function

      Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activation of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as histone chaperone that facilitates deposition of histone H3.3. Acts as targeting component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upon neuronal activation associates with regulatory elements of selected immediate early genes where it promotes deposition of histone H3.3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3.3:H4 dimers to PML-nuclear bodies (PML-NBs); the process is independent of ATRX and facilitated by ASF1A; PML-NBs are suggested to function as regulatory sites for the incorporation of newly synthesized histone H3.3 into chromatin. In case of overexpression of centromeric histone variant CENPA (as found in various tumors) is involved in its mislocalization to chromosomes; the ectopic localization involves a heterotypic tetramer containing CENPA, and histones H3.3 and H4 and decreases binding of CTCF to chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Shows restriction activity towards human cytomegalovirus (HCMV).
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Belongs to the DAXX family.
    • Domain

      The Sumo interaction motif mediates Sumo binding, and is required both for sumoylation and binding to sumoylated targets.
    • Post-translational
      modifications

      Sumoylated with SUMO1 on multiple lysine residues.
      Phosphorylated by HIPK1 upon glucose deprivation.
      Polyubiquitinated; which is promoted by CUL3 and SPOP and results in proteasomal degradation. Ubiquitinated by MDM2; inducing its degradation. Deubiquitinated by USP7; leading to stabilize it.
    • Cellular localization

      Nucleus. Diffuse nuclear distribution pattern and no comparable dot-like accumulation of isoform 1 and Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body. Nucleus, nucleolus. Chromosome, centromere. Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli (Probable). Colocalizes with histone H3.3, ATRX, HIRA and ASF1A at PML-nuclear bodies (PubMed:12953102, PubMed:14990586, PubMed:23222847, PubMed:24200965). Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres (PubMed:9645950). Detected in cytoplasmic punctate structures (PubMed:11842083). Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress (PubMed:12968034). Colocalizes with RASSF1 in the nucleus (PubMed:18566590). Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures (PubMed:16845383).
    • Target information above from: UniProt accession Q9UER7 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1616 Human
      • Omim: 603186 Human
      • SwissProt: Q9UER7 Human
      • Unigene: 336916 Human
      • Alternative names

        • BING 2 antibody
        • BING2 antibody
        • CENP-C binding protein antibody
        • DAP 6 antibody
        • DAP6 antibody
        • Daxx antibody
        • DAXX_HUMAN antibody
        • Death associated protein 6 antibody
        • Death domain associated protein 6 antibody
        • Death domain associated protein antibody
        • Death domain-associated protein 6 antibody
        • EAP 1 antibody
        • EAP1 antibody
        • ETS1 associated protein 1 antibody
        • ETS1-associated protein 1 antibody
        • Fas binding protein antibody
        • Fas death domain associated protein antibody
        • Fas death domain-associated protein antibody
        • hDaxx antibody
        • MGC126245 antibody
        • MGC126246 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-Daxx antibody (ab2017)
        Immunocytochemistry/ Immunofluorescence - Anti-Daxx antibody (ab2017)

        ab2017 at 10µg/ml staining DAXX in Hela cells by ICC/IF

      Protocols

      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab2017? Please let us know so that we can cite the reference in this datasheet.

      ab2017 has been referenced in 1 publication.

      • Dionne KR  et al. Daxx upregulation within the cytoplasm of reovirus-infected cells is mediated by interferon and contributes to apoptosis. J Virol 87:3447-60 (2013). PubMed: 23302889

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-3 of 3 Abreviews or Q&A

      Question

      what is the smallest amount (in nanogrammes) of protein you can detect with ab2017 in western blot analysis? does ab2017 antibody recognizes the denatured AND the native c-terminal part of daxx? does this antibody recognizes (non-specific) proteins from Hela cells? background?

      Read More

      Abcam community

      Verified customer

      Asked on Sep 06 2001

      Answer

      The sensitivity of Western blot is 1-10 ng of protein per band. We detected other antibodies but not this one. It detects the denatured protein, but we have not tested the native protein. It is possible to cross-recognize other proteins.

      Read More

      Neil Hayward

      Abcam Scientific Support

      Answered on Sep 11 2001

      Question

      Can we use this antibody in immunocytochemistry??

      Read More

      Abcam community

      Verified customer

      Asked on Dec 13 2000

      Answer

      This antibody has not been tested for use in immunocytochemistry. It has only been confirmed for Western Blot.

      Read More

      Abcam Scientific Support

      Answered on Dec 14 2000

      Question

      Does this antibody immunoprecipitates?

      Read More

      Abcam community

      Verified customer

      Asked on Nov 17 2000

      Answer

      It has not been tested for immunoprecipitation.

      Read More

      Abcam Scientific Support

      Answered on Nov 17 2000

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.