Overview

  • Product name

  • Description

    Rabbit polyclonal to DAZ2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 1 - 207 of Human DAZ2 (NP_065096).

  • Positive control

    • WB: 293T, A431, HeLa, MOLT4 and Raji cell lysate IF: HeLa cells

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab97772 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 63 kDa.
ICC/IF 1/100 - 1/200.

Target

  • Function

    RNA-binding protein that plays an essential role in spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation.
  • Tissue specificity

    Testis specific.
  • Involvement in disease

    Defects in DAZ2 may be a cause of spermatogenic failure Y-linked type 2 (SPGFY2) [MIM:415000]. It is a disorder resulting in the absence (azoospermia) or reduction (oligozoospermia) of sperm in the semen, leading to male infertility. Note=AZFc deletions in the Yq11.23 region including the DAZ genes are the most common known genetic cause of human male infertility.
  • Sequence similarities

    Belongs to the RRM DAZ family.
    Contains 15 DAZ-like domains.
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain

    The DAZ-like repeats are essential and mediate the interaction with DAZAP1 and DAZAP2.
  • Cellular localization

    Cytoplasm. Nucleus. Predominantly cytoplasmic. Nuclear at some stages of spermatozoide development. Localizes both to the nuclei and cytoplasm of spermatozoide differentiation. Nuclear in fetal gonocytes and in spermatogonial nuclei. It then relocates to the cytoplasm during male meiosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • DAZ2 antibody
    • DAZ2_HUMAN antibody
    • DAZ3 antibody
    • Deleted in azoospermia 2 antibody
    • Deleted in azoospermia protein 2 antibody
    • Deleted in azoospermia protein 3 antibody
    • MGC126442 antibody
    • pDP1678 antibody
    see all

Images

  • Anti-DAZ2 antibody (ab97772) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg

    Predicted band size: 63 kDa



    7.5% SDS PAGE
  • ab97772, at a 1/200 dilution, staining DAZ2 in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.

References

ab97772 has not yet been referenced specifically in any publications.

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