Key features and details
- Rabbit polyclonal to DAZAP1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DAZAP1 antibody
See all DAZAP1 primary antibodies
DescriptionRabbit polyclonal to DAZAP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human DAZAP1 aa 75-125. The exact sequence is proprietary. (GeneID 26528).
Database link: Q96EP5
- WB: HEK-293T, HeLa, Jurkat, TCMK-1 and NIH/3T3 cell lysates. IP: HEK-293T cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240603 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionRNA-binding protein, which may be required during spermatogenesis.
Tissue specificityMainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
- Information by UniProt
- DAZ associated protein 1 antibody
- DAZ-associated protein 1 antibody
- Dazap1 antibody
All lanes : Anti-DAZAP1 antibody (ab240603) at 0.4 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 10 seconds
DAZAP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab240603 at 6 μg/mL. For western blotting, ab240603 was used at 1 µg/ml.
Lane 1: ab240603 used for IP at 6 µg/mg per reaction.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240603 has not yet been referenced specifically in any publications.