Overview

  • Product name

  • Description

    Rabbit polyclonal to DAZAP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human DAZAP1 aa 75-125. The exact sequence is proprietary. (GeneID 26528).
    Database link: Q96EP5

  • Positive control

    • WB: HEK-293T, HeLa, Jurkat, TCMK-1 and NIH/3T3 cell lysates. IP: HEK-293T cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    RNA-binding protein, which may be required during spermatogenesis.
  • Tissue specificity

    Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • DAZ associated protein 1 antibody
    • DAZ-associated protein 1 antibody
    • Dazap1 antibody
    • DAZP1_HUMAN antibody
    • Deleted in azoospermia associated protein 1 antibody
    • Deleted in azoospermia-associated protein 1 antibody
    see all

Images

  • All lanes : Anti-DAZAP1 antibody (ab240603) at 0.4 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
    Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) cell lysate
    Lane 5 : NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Exposure time: 10 seconds
  • DAZAP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab240603 at 6 μg/mL. For western blotting, ab240603 was used at 1 µg/ml.

    Lane 1: ab240603 used for IP at 6 µg/mg per reaction.
    Lane 2: Control IgG.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

     

References

ab240603 has not yet been referenced specifically in any publications.

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