Key features and details
- Rabbit polyclonal to DAZAP1
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DAZAP1 antibody
See all DAZAP1 primary antibodies
DescriptionRabbit polyclonal to DAZAP1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human DAZAP1 aa 357-407. The exact sequence is proprietary. (GeneID 26528).
Database link: Q96EP5
- WB: HEK-293T, HeLa, Jurkat, TCMK-1 and NIH/3T3 cell lysate. IP: HEK-293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
Our Abpromise guarantee covers the use of ab240604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/1000 - 1/5000.|
FunctionRNA-binding protein, which may be required during spermatogenesis.
Tissue specificityMainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas.
Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm.
- Information by UniProt
- DAZ associated protein 1 antibody
- DAZ-associated protein 1 antibody
- Dazap1 antibody
DAZAP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab240604. For western blotting, ab240604 was used at 1 µg/ml.
Lane 1: ab240604 used for IP at 6 µg/mg lysate.
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 30 seconds.
All lanes : Anti-DAZAP1 antibody (ab240604) at 0.4 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 10 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240604 has not yet been referenced specifically in any publications.