Recombinant Anti-DAZAP1 antibody [EPR14400] (ab182558)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14400] to DAZAP1
- Suitable for: WB, Flow Cyt (Intra), IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DAZAP1 antibody [EPR14400]
See all DAZAP1 primary antibodies -
Description
Rabbit monoclonal [EPR14400] to DAZAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra), IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, HeLa and HepG2 cell lysates; Jurkat cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14400 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
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Flow Cyt (Intra) |
1/10.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IP |
1/50 - 1/170.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). |
Flow Cyt (Intra)
1/10. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IP
1/50 - 1/170. |
Target
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Function
RNA-binding protein, which may be required during spermatogenesis. -
Tissue specificity
Mainly expressed in testis. Expressed to a lower level in thymus. Weakly or not expressed in heart, liver, brain, placenta, lung, skeletal muscle, kidney and pancreas. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear at some stages of spermatozoides development. In midpachytene spermatocytes, it is localized in both the cytoplasm and the nuclei and is clearly excluded from the sex vesicles. In round spermatids, it localizes mainly in the nuclei, whereas in elongated spermatids, it localizes to the cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 26528 Human
- Entrez Gene: 70248 Mouse
- Entrez Gene: 362836 Rat
- Omim: 607430 Human
- SwissProt: Q96EP5 Human
- SwissProt: Q9JII5 Mouse
- Unigene: 222510 Human
- Unigene: 148693 Mouse
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Alternative names
- DAZ associated protein 1 antibody
- DAZ-associated protein 1 antibody
- Dazap1 antibody
see all
Images
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All lanes : Anti-DAZAP1 antibody [EPR14400] (ab182558) at 1/50000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa -
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling DAZAP1 with ab182558 at 1/10 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab182558 at 1/50 dilution (Lane 1). Lane 2: Negative control.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab182558 has been referenced in 1 publication.
- Chen Y et al. Starvation-induced suppression of DAZAP1 by miR-10b integrates splicing control into TSC2-regulated oncogenic autophagy in esophageal squamous cell carcinoma. Theranostics 10:4983-4996 (2020). PubMed: 32308763