Anti-DAZL antibody (ab34139)
Key features and details
- Rabbit polyclonal to DAZL
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human, Common marmoset
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-DAZL antibody
See all DAZL primary antibodies -
Description
Rabbit polyclonal to DAZL -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Common marmoset
Predicted to work with: Cow, Chimpanzee
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Immunogen
Synthetic peptide corresponding to Mouse DAZL aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab27733) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab34139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (6) |
Use a concentration of 0.5 - 5 µg/ml.
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| ICC/IF | (1) |
Use at an assay dependent concentration.
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| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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| Notes |
|---|
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IHC-P
Use a concentration of 0.5 - 5 µg/ml. |
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ICC/IF
Use at an assay dependent concentration. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
Target
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Function
RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts. -
Tissue specificity
Testis specific. -
Sequence similarities
Belongs to the RRM DAZ family.
Contains 1 DAZ-like domain.
Contains 1 RRM (RNA recognition motif) domain. -
Domain
The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward. - Information by UniProt
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Database links
- Entrez Gene: 460209 Chimpanzee
- Entrez Gene: 530116 Cow
- Entrez Gene: 1618 Human
- Entrez Gene: 13164 Mouse
- Omim: 601486 Human
- SwissProt: Q92904 Human
- SwissProt: Q64368 Mouse
- Unigene: 131179 Human
see all -
Alternative names
- DAZ homolog antibody
- DAZ like autosomal antibody
- DAZ-like autosomal antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody (ab34139)
IHC image of DAZL staining in a section of formalin-fixed paraffin-embedded normal mouse testis performed on a Leica BOND™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab34139, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Immunocytochemistry/ Immunofluorescence - Anti-DAZL antibody (ab34139)This image is courtesy of an Abreview submitted by Dr Sharon Sneddonab34139 at 1/100 staining human blastocyst cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 5% serum prior to incubation with the antibody for 18 hours. An Alexa-Fluor ® 488 conjugated secondary antibody was used.
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Immunocytochemistry/ Immunofluorescence - Anti-DAZL antibody (ab34139)The image, submitted as part of an Abreview, shows DAZL staining (green) of a human blastocyst. The antibody stains the cytoplasm of cells of the inner cell mass and trophoblast. DAZL is known to be located to the cytoplasm and DAZL transcripts have previously been discovered in the inner cell mass and trophblast (see PMID: 15879466). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody (ab34139)The image shows staining of human testis tissue. The cytoplasm of Leydig cells and other cells in the seminiferous tubules stained positive. The antigen retrieval techniques used were Citrate at pH6 or Tris EDTA at pH9. -
Western blot - Anti-DAZL antibody (ab34139)All lanes : Anti-DAZL antibody (ab34139) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Testis (Mouse) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 130 kDa, 70 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DAZL antibody (ab34139)This image is taken from an abreview by Zachary Yu-Ching Lin.Ab34139 staining DAZL on juvenile marmoset testes by immunohistochemistry. The tissue was paraformaldehyde fixed and blocked in 1% milk for 1 hour at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 9 hours at 4°C in 5% milk. An Alexa Fluor 555 conjugated goat anti-rabbit antibody was used as the secondary. Nuclear staining shown in blue with DAPI. -
Western blot - Anti-DAZL antibody (ab34139)Anti-DAZL antibody (ab34139) at 1 µg/ml + GST tagged Recombinant Human DAZL protein (ab112279) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Exposure time: 10 seconds
Protocols
Datasheets and documents
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Datasheet download
References (60)
ab34139 has been referenced in 60 publications.
- Zheng Y et al. Establishment of cell lines with porcine spermatogonial stem cell properties. J Anim Sci Biotechnol 11:33 (2020). PubMed: 32308978
- Gura MA et al. Dynamic and regulated TAF gene expression during mouse embryonic germ cell development. PLoS Genet 16:e1008515 (2020). PubMed: 31914128
- Zhou Y et al. Puerarin improves graft bone defect through microRNA-155-3p-mediated p53/TNF-a/STAT1 signaling pathway. Int J Mol Med 46:239-251 (2020). PubMed: 32377717
- Zhang F et al. Suppressing Dazl modulates tumorigenicity and stemness in human glioblastoma cells. BMC Cancer 20:673 (2020). PubMed: 32682409
- Mikedis MM et al. DAZL mediates a broad translational program regulating expansion and differentiation of spermatogonial progenitors. Elife 9:N/A (2020). PubMed: 32686646
