Overview

  • Product name

    Anti-DAZL antibody [EPR21028]
    See all DAZL primary antibodies
  • Description

    Rabbit monoclonal [EPR21028] to DAZL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Horse, Cow, Pig
  • Immunogen

    Recombinant fragment within Mouse DAZL aa 1-300. The exact sequence is proprietary.
    Database link: Q64368

  • Positive control

    • WB: Human, mouse and rat testis lysates. IHC-P: Human, mouse and rat testis tissues. IHC-Fr: Mouse and rat testis tissues. IP: Mouse testis lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

(Human); 1/4000 (Mouse and rat).

IHC-Fr 1/300.

(Mouse); 1/60 (Rat).

Perform heat mediated antigen retrieval by using sodium citrate buffer (pH 6.0).

IP 1/30.

Target

  • Function

    RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
  • Tissue specificity

    Testis specific.
  • Sequence similarities

    Belongs to the RRM DAZ family.
    Contains 1 DAZ-like domain.
    Contains 1 RRM (RNA recognition motif) domain.
  • Domain

    The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.
  • Cellular localization

    Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.
  • Information by UniProt
  • Database links

  • Alternative names

    • DAZ homolog antibody
    • DAZ like autosomal antibody
    • DAZ-like autosomal antibody
    • DAZH antibody
    • DAZL 1 antibody
    • DAZL antibody
    • DAZL_HUMAN antibody
    • DAZL1 antibody
    • DAZLA antibody
    • Deleted in azoospermia like 1 antibody
    • Deleted in azoospermia like antibody
    • Deleted in azoospermia like autosomal antibody
    • Deleted in azoospermia-like 1 antibody
    • Deleted in azoospermia-like antibody
    • Germline specific RNA binding protein antibody
    • MGC26406 antibody
    • Spermatogenesis gene on the Y like autosomal antibody
    • SPGY like autosomal antibody
    • SPGY-like-autosomal antibody
    • SPGYLA antibody
    • Tpx2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human testis tissue labeling DAZL with ab215718 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in primary spermatocytes of human testis (PMID: 24746554) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen mouse testis tissue labeling DAZL with ab215718 at 1/300 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in the pachytene spermatocytes and secondary spermatocytes, not present in Sertoli, Leydig or myoid cells (PMID: 24746554).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • All lanes : Anti-DAZL antibody [EPR21028] (ab215718) at 1/1000 dilution

    Lane 1 : Mouse testis tissue lysate
    Lane 2 : DAZL knockout mouse testis tissue lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP-conjugated horse anti-rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 33 kDa
    Observed band size: 37 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 seconds


    10% gel.

    Blocked with 5% milk for 1 hour at room temperature.

    Incubated with the primary antibody for 12 hours at 4°C in TBST.

    See Abreview

  • All lanes : Anti-DAZL antibody [EPR21028] (ab215718) at 1/1000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Rat testis lysate
    Lane 3 : Human testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 33 kDa
    Observed band size: 38 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

  • DAZL was immunoprecipitated from 0.35 mg mouse testis lysate with ab215718 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215718 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse testis lysate 10 μg (Input).

    Lane 2: ab215718 IP in mouse testis lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of  ab215718 in mouse testis lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling DAZL with ab215718 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in spermatogonia and primary spermatocytes of mouse testis (PMID: 24746554) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling DAZL with ab215718 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in spermatogonia and primary spermatocytes of rat testis (PMID: 24746554) is observed. Counterstained with hematoxylin

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton-X-100 permeabilized frozen rat testis tissue labeling DAZL with ab215718 at 1/60 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Cytoplasmic staining in the pachytene spermatocytes and secondary spermatocytes, not present in Sertoli, Leydig or myoid cells (PMID: 24746554).

    The nuclear counter stain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

References

This product has been referenced in:

  • Huss DJ  et al. Avian Primordial Germ Cells Contribute to and Interact With the Extracellular Matrix During Early Migration. Front Cell Dev Biol 7:35 (2019). Read more (PubMed: 30984757) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse Testis)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
25 µg
Specification
mouse Testis
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 08 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Aug 23 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Aug 23 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Testis)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Testis
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Aug 20 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Mr. Bryan Niedenberger

Verified customer

Submitted Jan 30 2018

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