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RecombinantRabMAb

Recombinant Anti-DAZL antibody [EPR5140] (ab108372)

  • Datasheet
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Western blot - Anti-DAZL antibody [EPR5140] (ab108372)
  • Anti-DAZL antibody [EPR5140] (ab108372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5140] to DAZL
  • Suitable for: WB
  • Reacts with: Human

You may also be interested in

Protein
Product image
Recombinant Human DAZL protein (ab112279)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-DAZL antibody [EPR5140]
    See all DAZL primary antibodies
  • Description

    Rabbit monoclonal [EPR5140] to DAZL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human DAZL aa 1-100. The exact sequence is proprietary.

  • Positive control

    • Human testis and Human small intestine lysates
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5140
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Developmental Biology
    • Reproduction
    • Germ cell markers

Associated products

  • Alternative Versions

    • Anti-DAZL antibody [EPR5140] - BSA and Azide free (ab247651)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human DAZL protein (ab112279)

Applications

Our Abpromise guarantee covers the use of ab108372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
    • Tissue specificity

      Testis specific.
    • Sequence similarities

      Belongs to the RRM DAZ family.
      Contains 1 DAZ-like domain.
      Contains 1 RRM (RNA recognition motif) domain.
    • Domain

      The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.
    • Cellular localization

      Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.
    • Target information above from: UniProt accession Q92904 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1618 Human
      • Entrez Gene: 13164 Mouse
      • Omim: 601486 Human
      • SwissProt: Q92904 Human
      • SwissProt: Q64368 Mouse
      • Unigene: 131179 Human
      • Unigene: 280641 Mouse
      • Alternative names

        • DAZ homolog antibody
        • DAZ like autosomal antibody
        • DAZ-like autosomal antibody
        • DAZH antibody
        • DAZL 1 antibody
        • DAZL antibody
        • DAZL_HUMAN antibody
        • DAZL1 antibody
        • DAZLA antibody
        • Deleted in azoospermia like 1 antibody
        • Deleted in azoospermia like antibody
        • Deleted in azoospermia like autosomal antibody
        • Deleted in azoospermia-like 1 antibody
        • Deleted in azoospermia-like antibody
        • Germline specific RNA binding protein antibody
        • MGC26406 antibody
        • Spermatogenesis gene on the Y like autosomal antibody
        • SPGY like autosomal antibody
        • SPGY-like-autosomal antibody
        • SPGYLA antibody
        • Tpx2 antibody
        see all

      Images

      • Western blot - Anti-DAZL antibody [EPR5140] (ab108372)
        Western blot - Anti-DAZL antibody [EPR5140] (ab108372)
        All lanes : Anti-DAZL antibody [EPR5140] (ab108372) at 1/1000 dilution

        Lane 1 : Human testis lysate
        Lane 2 : Human small intestine lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 33 kDa

      • Anti-DAZL antibody [EPR5140] (ab108372)
        Anti-DAZL antibody [EPR5140] (ab108372)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab108372? Please let us know so that we can cite the reference in this datasheet.

      ab108372 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Immunohistochemistry (Frozen sections) abreview for Anti-DAZL antibody [EPR5140]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Testis, adult)
      Permeabilization
      Yes - 0.1% Triton X-100 in PBS
      Specification
      Testis, adult
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More

      Mr. Bryan Niedenberger

      Verified customer

      Submitted Mar 11 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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