Recombinant
RabMAb

Anti-DAZL antibody [EPR5140] (ab108372)

Abreviews (1) Submit a Question

Overview

  • Product name
    Anti-DAZL antibody [EPR5140]
    See all DAZL primary antibodies
  • Description
    Rabbit monoclonal [EPR5140] to DAZL
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human DAZL aa 1-100. The exact sequence is proprietary.

  • Positive control
    • Human testis and Human small intestine lysates
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab108372 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.

    • Target

    • Function
      RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts.
    • Tissue specificity
      Testis specific.
    • Sequence similarities
      Belongs to the RRM DAZ family.
      Contains 1 DAZ-like domain.
      Contains 1 RRM (RNA recognition motif) domain.
    • Domain
      The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2.
    • Cellular localization
      Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward.
    • Information by UniProt
    • Database links
      • Alternative names
        • DAZ homolog antibody
        • DAZ like autosomal antibody
        • DAZ-like autosomal antibody
        • DAZH antibody
        • DAZL 1 antibody
        • DAZL antibody
        • DAZL_HUMAN antibody
        • DAZL1 antibody
        • DAZLA antibody
        • Deleted in azoospermia like 1 antibody
        • Deleted in azoospermia like antibody
        • Deleted in azoospermia like autosomal antibody
        • Deleted in azoospermia-like 1 antibody
        • Deleted in azoospermia-like antibody
        • Germline specific RNA binding protein antibody
        • MGC26406 antibody
        • Spermatogenesis gene on the Y like autosomal antibody
        • SPGY like autosomal antibody
        • SPGY-like-autosomal antibody
        • SPGYLA antibody
        • Tpx2 antibody
        see all

      Images

      • Western blot - Anti-DAZL antibody [EPR5140] (ab108372)
        Western blot - Anti-DAZL antibody [EPR5140] (ab108372)
        All lanes : Anti-DAZL antibody [EPR5140] (ab108372) at 1/1000 dilution

        Lane 1 : Human testis lysate
        Lane 2 : Human small intestine lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 33 kDa

      References

      ab108372 has not yet been referenced specifically in any publications.

      Publishing research using ab108372? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Submit a review Submit a question

      1 Review or Q&A

      10
      Votes: Highest First

      Immunohistochemistry (Frozen sections) abreview for Anti-DAZL antibody [EPR5140]

       Poor
      Abreviews
      Abcam has not validated the combination of species/application used in this Abreview.
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Testis, adult)
      Permeabilization
      Yes - 0.1% Triton X-100 in PBS
      Specification
      Testis, adult
      Blocking step
      BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
      Fixative
      Paraformaldehyde
      Read More
      abreview image
      Username

      Mr. Bryan Niedenberger

      Verified customer

      Submitted Mar 11 2016

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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