Recombinant Anti-DAZL antibody [EPR5140] (ab108372)
![Western blot - Anti-DAZL antibody [EPR5140] (ab108372)](https://www.abcam.com/ps/products/108/ab108372/Images/ab108372-121011-anti-dazl-antibody-epr5140-western-blot.jpg "Western blot - Anti-DAZL antibody [EPR5140] (ab108372)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5140] to DAZL
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DAZL antibody [EPR5140]
See all DAZL primary antibodies -
Description
Rabbit monoclonal [EPR5140] to DAZL -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human testis and Human small intestine lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5140 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Target
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Function
RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts. -
Tissue specificity
Testis specific. -
Sequence similarities
Belongs to the RRM DAZ family.
Contains 1 DAZ-like domain.
Contains 1 RRM (RNA recognition motif) domain. -
Domain
The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward. - Information by UniProt
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Database links
- Entrez Gene: 1618 Human
- Entrez Gene: 13164 Mouse
- Omim: 601486 Human
- SwissProt: Q92904 Human
- SwissProt: Q64368 Mouse
- Unigene: 131179 Human
- Unigene: 280641 Mouse
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Alternative names
- DAZ homolog antibody
- DAZ like autosomal antibody
- DAZ-like autosomal antibody
see all
Images
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Western blot - Anti-DAZL antibody [EPR5140] (ab108372)All lanes : Anti-DAZL antibody [EPR5140] (ab108372) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa -
![Anti-DAZL antibody [EPR5140] (ab108372)](https://www.abcam.com/ps/products/108/ab108372/Images/ab108372-2-benefits-of-recombinant-antibodies.png)
Anti-DAZL antibody [EPR5140] (ab108372)
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab108372 has been referenced in 1 publication.
- Feng Z et al. Adaptive Autophagy Offers Cardiorenal Protection in Rats with Acute Myocardial Infarction. Cardiol Res Pract 2020:7158975 (2020). PubMed: 32655948