Recombinant Anti-DAZL antibody [EPR5140] (ab108372)
![Western blot - Anti-DAZL antibody [EPR5140] (ab108372)](https://www.abcam.com/ps/products/108/ab108372/Images/ab108372-121011-anti-dazl-antibody-epr5140-western-blot.jpg "Western blot - Anti-DAZL antibody [EPR5140] (ab108372)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5140] to DAZL
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-DAZL antibody [EPR5140]
See all DAZL primary antibodies -
Description
Rabbit monoclonal [EPR5140] to DAZL -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide within Human DAZL aa 1-100. The exact sequence is proprietary.
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Positive control
- Human testis and Human small intestine lysates
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5140 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
Target
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Function
RNA-binding protein, which is essential for gametogenesis. Plays a central role during spermatogenesis. May act by binding to the 3'-UTR of mRNA and thereby regulating the translation of key transcripts. -
Tissue specificity
Testis specific. -
Sequence similarities
Belongs to the RRM DAZ family.
Contains 1 DAZ-like domain.
Contains 1 RRM (RNA recognition motif) domain. -
Domain
The DAZ-like domain mediates the interaction with DAZAP1 and DAZAP2. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic (By similarity). Nuclear in spermatogonia until near the end of the meiotic prophase and cytoplasmic localization from then onward. - Information by UniProt
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Database links
- Entrez Gene: 1618 Human
- Entrez Gene: 13164 Mouse
- Omim: 601486 Human
- SwissProt: Q92904 Human
- SwissProt: Q64368 Mouse
- Unigene: 131179 Human
- Unigene: 280641 Mouse
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Alternative names
- DAZ homolog antibody
- DAZ like autosomal antibody
- DAZ-like autosomal antibody
see all
Images
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Western blot - Anti-DAZL antibody [EPR5140] (ab108372)All lanes : Anti-DAZL antibody [EPR5140] (ab108372) at 1/1000 dilution
Lane 1 : Human testis lysate
Lane 2 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 33 kDa -
![Anti-DAZL antibody [EPR5140] (ab108372)](https://www.abcam.com/ps/products/108/ab108372/Images/ab108372-2-benefits-of-recombinant-antibodies.png)
Anti-DAZL antibody [EPR5140] (ab108372)
Datasheets and documents
References (0)
ab108372 has not yet been referenced specifically in any publications.
