Overview

  • Product name

  • Description

    Rabbit polyclonal to DB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human DB1 aa 500 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab129953)

  • Positive control

    • This antibody gave a positive signal in both Human Skeletal Muscle and Human Kidney tissue lysates.
  • General notes

    Previously labelled as ZNF161. 

Properties

Applications

Our Abpromise guarantee covers the use of ab68278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • Function

    Possible transcription factor. Specifically binds to the CT/GC-rich region of the interleukin-3 promoter and mediates tax transactivation of IL-3.
  • Tissue specificity

    Ubiquitously expressed. Highest levels in skeletal muscle and kidney.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DB1 antibody
    • MGC82370 antibody
    • Putative transcription factor DB1 antibody
    • Vascular endothelial zinc finger 1 antibody
    • VEZF1 antibody
    • VEZF1_HUMAN antibody
    • Zinc finger protein 161 antibody
    • ZNF161 antibody
    see all

Images

  • All lanes : Anti-DB1 antibody (ab68278) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 29 kDa, 48 kDa, 87 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes

References

ab68278 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab68278.
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