Anti-DB1 antibody [ZNF5J141] (ab50970)
Key features and details
- Mouse monoclonal [ZNF5J141] to DB1
- Suitable for: WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-DB1 antibody [ZNF5J141]
See all DB1 primary antibodies -
Description
Mouse monoclonal [ZNF5J141] to DB1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human DB1.
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Positive control
- HeLa cells and whole cell lysate and NIH3T3 whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.0225% Potassium chloride, 0.0204% Dibasic monohydrogen potassium phosphate, 0.1136% Dibasic monohydrogen sodium phosphate, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab50970 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. -
Clonality
Monoclonal -
Clone number
ZNF5J141 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/50. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
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ICC |
1/1.
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Notes |
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WB
1/50. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa). |
ICC
1/1. |
Target
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Function
Possible transcription factor. Specifically binds to the CT/GC-rich region of the interleukin-3 promoter and mediates tax transactivation of IL-3. -
Tissue specificity
Ubiquitously expressed. Highest levels in skeletal muscle and kidney. -
Sequence similarities
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7716 Human
- Entrez Gene: 22344 Mouse
- Omim: 606747 Human
- SwissProt: Q14119 Human
- Unigene: 463569 Human
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Alternative names
- DB1 antibody
- MGC82370 antibody
- Putative transcription factor DB1 antibody
see all
Images
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Anti-DB1 antibody [ZNF5J141] (ab50970) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
ab50970 at 1/1 dilution staining human DB1 in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat Anti-mouse IgG at 1/200 as secondary.
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Anti-DB1 antibody [ZNF5J141] (ab50970) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab50970 has been referenced in 2 publications.
- Fu J et al. Inhibition of miR-495 Improves Both Vascular Remodeling and Angiogenesis in Pulmonary Hypertension. J Vasc Res 56:97-106 (2019). PubMed: 31030195
- Gerald D et al. RhoB controls coordination of adult angiogenesis and lymphangiogenesis following injury by regulating VEZF1-mediated transcription. Nat Commun 4:2824 (2013). WB . PubMed: 24280686