Overview

  • Product name

    Anti-DB1 antibody [ZNF5J141]
    See all DB1 primary antibodies
  • Description

    Mouse monoclonal [ZNF5J141] to DB1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC, Dot blotmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human DB1.

  • Positive control

    • HeLa cells and whole cell lysate and NIH3T3 whole cell lysate
  • General notes

    Previously labelled as ZNF161. 

Properties

Applications

Our Abpromise guarantee covers the use of ab50970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
ICC 1/1.
Dot blot Use at an assay dependent dilution.

Target

  • Function

    Possible transcription factor. Specifically binds to the CT/GC-rich region of the interleukin-3 promoter and mediates tax transactivation of IL-3.
  • Tissue specificity

    Ubiquitously expressed. Highest levels in skeletal muscle and kidney.
  • Sequence similarities

    Belongs to the krueppel C2H2-type zinc-finger protein family.
    Contains 6 C2H2-type zinc fingers.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DB1 antibody
    • MGC82370 antibody
    • Putative transcription factor DB1 antibody
    • Vascular endothelial zinc finger 1 antibody
    • VEZF1 antibody
    • VEZF1_HUMAN antibody
    • Zinc finger protein 161 antibody
    • ZNF161 antibody
    see all

Images

  • Anti-DB1 antibody [ZNF5J141] (ab50970) at 1/50 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 57 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • ab50970 at 1/1 dilution staining human DB1 in HeLa cells by Immunocytochemistry. AlexaFluor 488 Goat Anti-mouse IgG at 1/200 as secondary.

  • Anti-DB1 antibody [ZNF5J141] (ab50970) at 1/50 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    anti-mouse IgG at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 57 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Gerald D  et al. RhoB controls coordination of adult angiogenesis and lymphangiogenesis following injury by regulating VEZF1-mediated transcription. Nat Commun 4:2824 (2013). WB . Read more (PubMed: 24280686) »
See 1 Publication for this product

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