Key features and details
- Rabbit polyclonal to DBC-1
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-DBC-1 antibody
See all DBC-1 primary antibodies
DescriptionRabbit polyclonal to DBC-1
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide corresponding to Human DBC-1 aa 875-923 (C terminal).
Database link: Q8N163
- Nuclear extract from HeLa cells
Previously labelled as KIAA1967.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Tris-citrate/phosphate buffer, pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).|
|IP||Use at 1-10 µg/mg of lysate.|
FunctionCore component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity.
Tissue specificityExpressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.
- Information by UniProt
- CCAR2 antibody
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Lanes 1-2 : Anti-DBC-1 antibody (ab70242) at 1/1000 dilution
Lanes 3-4 : Anti-DBC-1 antibody (ab70242) at 1/10000 dilution
Lanes 1 & 3 : Nuclear extract from HeLa cells at 15 µg
Lanes 2 & 4 : Nuclear extract from HeLa cells at 45 µg
Developed using the ECL technique.
Predicted band size: 103 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DBC-1 with ab70242 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation: ab70242 used at 1 µg/100 µg of nuclear extract from HeLa cells. Western blot: 1/1000 ab70242. Detection: Chemiluminescence with exposure times of 5 mins
ab70242 has been referenced in 1 publication.
- Raynes R et al. The SIRT1 modulators AROS and DBC1 regulate HSF1 activity and the heat shock response. PLoS One 8:e54364 (2013). WB ; Human . PubMed: 23349863