Overview

  • Product name

  • Description

    Rabbit polyclonal to DBC-1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human DBC-1 aa 875-923 (C terminal).
    Database link: Q8N163

  • Positive control

    • Nuclear extract from HeLa cells
  • General notes

    Previously labelled as KIAA1967. 

Properties

Applications

Our Abpromise guarantee covers the use of ab70242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 103 kDa).
IP Use at 1-10 µg/mg of lysate.

Target

  • Function

    Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity.
  • Tissue specificity

    Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCAR2 antibody
    • Cell cycle and apoptosis regulator 2 antibody
    • Cell division cycle and apoptosis regulator protein 2 antibody
    • DBC-1 antibody
    • DBC.1 antibody
    • DBC1 antibody
    • DBIRD complex subunit KIAA1967 antibody
    • Deleted in breast cancer 1 antibody
    • Deleted in breast cancer gene 1 protein antibody
    • K1967_HUMAN antibody
    • KIAA1967 antibody
    • NET35 antibody
    • p30 DBC antibody
    • p30 DBC protein antibody
    • p30DBC antibody
    see all

Images

  • Lanes 1-2 : Anti-DBC-1 antibody (ab70242) at 1/1000 dilution
    Lanes 3-4 : Anti-DBC-1 antibody (ab70242) at 1/10000 dilution

    Lanes 1 & 3 : Nuclear extract from HeLa cells at 15 µg
    Lanes 2 & 4 : Nuclear extract from HeLa cells at 45 µg

    Developed using the ECL technique.

    Predicted band size: 103 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DBC-1 with ab70242 at 1/1000 (1µg/ml). Detection: DAB.

  • Immunoprecipitation: ab70242 used at 1 µg/100 µg of nuclear extract from HeLa cells. Western blot: 1/1000 ab70242. Detection: Chemiluminescence with exposure times of 5 mins

References

This product has been referenced in:

  • Raynes R  et al. The SIRT1 modulators AROS and DBC1 regulate HSF1 activity and the heat shock response. PLoS One 8:e54364 (2013). WB ; Human . Read more (PubMed: 23349863) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab70242.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up