Recombinant
RabMAb

Recombinant Anti-DBC-1 antibody [EPR19747] (ab215852)

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Overview

  • Product name

    Anti-DBC-1 antibody [EPR19747]
    See all DBC-1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19747] to DBC-1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human DBC-1 aa 900 to the C-terminus. The exact sequence is proprietary.
    Database link: Q8N163

  • Positive control

    • WB: HeLa, 293 and Jurkat whole cell lysates; Human lung cancer, breast cancer and fetal kidney lysates. IHC-P: Human colon, cervix cancer and colon cancer tissues. ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: HeLa whole cell lysate.

  • General notes

    Previously labelled as KIAA1967. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40.
ICC/IF 1/500.
Flow Cyt 1/700.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 102 kDa).

Target

  • Function

    Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity.

  • Tissue specificity

    Expressed ubiquitously in normal tissues. Expressed in 84 to 100% of neoplastic breast, lung, and colon tissues.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • CCAR2 antibody
      • Cell cycle and apoptosis regulator 2 antibody
      • Cell division cycle and apoptosis regulator protein 2 antibody
      • DBC-1 antibody
      • DBC.1 antibody
      • DBC1 antibody
      • DBIRD complex subunit KIAA1967 antibody
      • Deleted in breast cancer 1 antibody
      • Deleted in breast cancer gene 1 protein antibody
      • K1967_HUMAN antibody
      • KIAA1967 antibody
      • NET35 antibody
      • p30 DBC antibody
      • p30 DBC protein antibody
      • p30DBC antibody
      see all

    Images

    • Western blot - Anti-DBC-1 antibody [EPR19747] (ab215852)
      Western blot - Anti-DBC-1 antibody [EPR19747] (ab215852)
      All lanes : Anti-DBC-1 antibody [EPR19747] (ab215852) at 1/1000 dilution

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : 293 (Human epithelial cell line from embryonic kidney) whole cell lysate
      Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 102 kDa
      Observed band size: 130 kDa
      why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1/2: 3 minutes; Lane 3: 10 seconds.

    • Immunocytochemistry/ Immunofluorescence - Anti-DBC-1 antibody [EPR19747] (ab215852)
      Immunocytochemistry/ Immunofluorescence - Anti-DBC-1 antibody [EPR19747] (ab215852)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling DBC-1 with ab215852 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

      Confocal image showing nuclear staining on HeLa cell line.

      The nuclear counterstain is DAPI (blue).

      Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody [EPR19747] (ab215852)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody [EPR19747] (ab215852)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling DBC-1 with ab215852 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nuclear staining on human colon is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry - Anti-DBC-1 antibody [EPR19747] (ab215852)
      Flow Cytometry - Anti-DBC-1 antibody [EPR19747] (ab215852)

      Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling DBC-1 with ab215852 at 1/700 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    • Immunoprecipitation - Anti-DBC-1 antibody [EPR19747] (ab215852)
      Immunoprecipitation - Anti-DBC-1 antibody [EPR19747] (ab215852)

      DBC-1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cells from cervix adenocarcinoma cell line) whole cell lysate with ab215852 at 1/40 dilution.

      Western blot was performed from the immunoprecipitate using ab215852 at 1/1000 dilution.

      VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

      Lane 1: HeLa whole cell lysate, 10µg (Input).

      Lane 2: ab215852 IP in HeLa whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215852 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 minutes.

    • Western blot - Anti-DBC-1 antibody [EPR19747] (ab215852)
      Western blot - Anti-DBC-1 antibody [EPR19747] (ab215852)
      All lanes : Anti-DBC-1 antibody [EPR19747] (ab215852) at 1/1000 dilution

      Lane 1 : Human lung cancer lysate
      Lane 2 : Human breast cancer lysate
      Lane 3 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

      Predicted band size: 102 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?



      Blocking/Dilution buffer: 5% NFDM/TBST.

      Exposure time: Lane 1/2: 3 minutes; Lane 3: 3 seconds.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody [EPR19747] (ab215852)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody [EPR19747] (ab215852)

      Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling DBC-1 with ab215852 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nuclear staining on human cervix cancer is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody [EPR19747] (ab215852)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DBC-1 antibody [EPR19747] (ab215852)

      Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling DBC-1 with ab215852 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Nuclear staining on human colon cancer is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    ab215852 has not yet been referenced specifically in any publications.

    Publishing research using ab215852? Please let us know so that we can cite the reference in this datasheet.

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