Overview

  • Product name

  • Description

    Rabbit polyclonal to DBF4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human DBF4 aa 101-281.
    Database link: Q9UBU7

  • Positive control

    • WB: HepG2, K562 and HeLa cell lysate. IHC-P: Human testis tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab235099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
WB 1/500 - 1/5000.

Target

  • Function

    Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
  • Tissue specificity

    Highly expressed in testis and thymus. Expressed also in most cancer cells lines.
  • Sequence similarities

    Contains 2 BRCT domains.
    Contains 1 DBF4-type zinc finger.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activator of S phase kinase antibody
    • ASK antibody
    • CHIF antibody
    • Chiffon homolog A antibody
    • DBF4 antibody
    • DBF4 homolog (S. cerevisiae) antibody
    • DBF4 homolog antibody
    • DBF4 type zinc finger containing protein 1 antibody
    • DBF4 zinc finger A antibody
    • DBF4 zinc finger antibody
    • DBF4, S. cerevisiae, homolog of antibody
    • DBF4-type zinc finger-containing protein 1 antibody
    • DBF4A antibody
    • DBF4A_HUMAN antibody
    • Dumbbell former 4, S. cerevisiae, homolog of antibody
    • OTTHUMP00000161661 antibody
    • Protein DBF4 homolog A antibody
    • ZDBF1 antibody
    • Zinc finger, DBF-type containing 1 antibody
    see all

Images

  • All lanes : Anti-DBF4 antibody (ab235099) at 1/500 dilution

    Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
    Lane 2 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
  • Paraffin-embedded human testis tissue stained for DBF4 with ab235099 (1/100 dilution) in immunohistochemical analysis.

References

ab235099 has not yet been referenced specifically in any publications.

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