Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5635] to DBF4
- Suitable for: WB
- Reacts with: Human
Product nameAnti-DBF4 antibody [EPR5635]
See all DBF4 primary antibodies
DescriptionRabbit monoclonal [EPR5635] to DBF4
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to residues in Human DBF4 protein (Q9UBU7).
- PC-3, Jurkat, SW480, and HepG2 whole cell lysate (ab7900).
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.49 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 77 kDa.|
FunctionRegulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
Tissue specificityHighly expressed in testis and thymus. Expressed also in most cancer cells lines.
Sequence similaritiesContains 2 BRCT domains.
Contains 1 DBF4-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- Activator of S phase kinase antibody
- ASK antibody
- CHIF antibody
All lanes : Anti-DBF4 antibody [EPR5635] (ab124707) at 1/10000 dilution
Lane 1 : PC-3 cell lysates
Lane 2 : Jurkat cell lysates
Lane 3 : SW480 cell lysates
Lane 4 : HepG2 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 77 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab124707 has been referenced in 4 publications.
- Iwai K et al. Molecular mechanism and potential target indication of TAK-931, a novel CDC7-selective inhibitor. Sci Adv 5:eaav3660 (2019). PubMed: 31131319
- Chen EW et al. A Dual Inhibitor of Cdc7/Cdk9 Potently Suppresses T Cell Activation. Front Immunol 10:1718 (2019). PubMed: 31402912
- Li X et al. Nuclear PGK1 Alleviates ADP-Dependent Inhibition of CDC7 to Promote DNA Replication. Mol Cell 72:650-660.e8 (2018). PubMed: 30392930
- Knockleby J et al. Cdk1-mediated phosphorylation of Cdc7 suppresses DNA re-replication. Cell Cycle 15:1494-505 (2016). PubMed: 27105124