Overview

  • Product name

  • Description

    Rabbit polyclonal to DBT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 11-247 of Human DBT (P11182).

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, Molt4, Raji whole cell lysates; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab151991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 53 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    DBT or dihydrolipoamide branched chain transacylase E2 is a component of the BCKD (branched-chain alpha-keto dehydrogenase) complex which is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. The BCKD complex is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine.
  • Cellular localization

    Mitochondrion matrix.
  • Database links

  • Alternative names

    • BCATE2 antibody
    • BCKAD E2 subunit antibody
    • Branched chain acyltransferase, E2 component antibody
    • Dihydrolipoamide branched chain transacylase (E2 component of branched chain keto acid dehydrogenase complex; maple syrup urine disease) antibody
    • Dihydrolipoamide branched chain transacylase antibody
    • Dihydrolipoamide branched chain transacylase E2 antibody
    • Dihydrolipoyl transacylase antibody
    • Dihydrolipoyllysine residue (2 methylpropanoyl)transferase antibody
    • E2 antibody
    • E2 component of branched chain alpha keto acid dehydrogenase complex antibody
    • E2B antibody
    • Lipoamide acyltransferase component of mitochondrial branched chain alpha keto acid dehydrogenase complex antibody
    • Mitochondrial branched chain alpha keto acid dehydrogenase transacylase subunit (E2b) antibody
    see all

Images

  • All lanes : Anti-DBT antibody (ab151991) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 53 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol fixed A431 cells labeling DBT with ab151991 at 1/200. Lower panel shows costaining with Hoechst 33342.

References

ab151991 has not yet been referenced specifically in any publications.

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