Anti-DC-SIGN antibody (ab5715)
Key features and details
- Rabbit polyclonal to DC-SIGN
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-DC-SIGN antibody
See all DC-SIGN primary antibodies -
Description
Rabbit polyclonal to DC-SIGN -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human DC-SIGN aa 384-404.
(Peptide available asab6091) -
Positive control
- Human placenta tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5715 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 10 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa.
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Notes |
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ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 44 kDa. |
Target
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Function
Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells. -
Tissue specificity
Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. -
Sequence similarities
Contains 1 C-type lectin domain. -
Domain
The tandem repeat domain, also called neck domain, mediates oligomerization. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 30835 Human
- Omim: 604672 Human
- SwissProt: Q9NNX6 Human
- Unigene: 278694 Human
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Alternative names
- C type lectin domain family 4 member L antibody
- C-type lectin domain family 4 member L antibody
- CD 209 antibody
see all
Images
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Immunohistochemistry of DC-SIGN in human lymph node tissue with DC-SIGN antibody at 10 µg/ml.
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ab5715 at 0.5
µ g /ml staining approximately 44kDa of human CD209 in Human placenta lysate by Western blot (ECL). -
Lane 1 : Anti-DC-SIGN antibody (ab5715) at 1 µg/ml
Lane 2 : Anti-DC-SIGN antibody (ab5715) at 2 µg/ml
All lanes : Human placenta
Observed band size: 44 kDa why is the actual band size different from the predicted? -
Immunofluorescence of DC-SIGN in Human Lymph Node cells using ab5715 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab5715 has been referenced in 4 publications.
- Xiaocui L et al. CSF-1-induced DC-SIGN+ macrophages are present in the ovarian endometriosis. Reprod Biol Endocrinol 20:48 (2022). PubMed: 35260161
- Singh S et al. Carbohydrates from Pseudomonas aeruginosa biofilms interact with immune C-type lectins and interfere with their receptor function. NPJ Biofilms Microbiomes 7:87 (2021). PubMed: 34880222
- Green KY et al. Human norovirus targets enteroendocrine epithelial cells in the small intestine. Nat Commun 11:2759 (2020). PubMed: 32488028
- Moutsopoulos NM et al. Defective neutrophil recruitment in leukocyte adhesion deficiency type I disease causes local IL-17-driven inflammatory bone loss. Sci Transl Med 6:229ra40 (2014). PubMed: 24670684