Anti-DC-SIGN antibody [C209/1781] (ab218419)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)](https://www.abcam.com/ps/products/218/ab218419/Images/ab218419-274087-anti-dc-sign-antibody-c209-1781-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)")
Key features and details
- Mouse monoclonal [C209/1781] to DC-SIGN
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-DC-SIGN antibody [C209/1781]
See all DC-SIGN primary antibodies -
Description
Mouse monoclonal [C209/1781] to DC-SIGN -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human DC-SIGN. The exact sequence is proprietary.
Database link: Q9NNX6 -
Positive control
- IHC-P: Human lung carcinoma, colon carcinoma and small intestine tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
C209/1781 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab218419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells. -
Tissue specificity
Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes. -
Sequence similarities
Contains 1 C-type lectin domain. -
Domain
The tandem repeat domain, also called neck domain, mediates oligomerization. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 30835 Human
- Omim: 604672 Human
- SwissProt: Q9NNX6 Human
- Unigene: 278694 Human
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Alternative names
- C type lectin domain family 4 member L antibody
- C-type lectin domain family 4 member L antibody
- CD 209 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)
Immunohistochemical analysis of paraffin embedded, formalin fixed human small intestine tissue labeling DC-SIGN using ab218419 at 1 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)](https://www.abcam.com/ps/products/218/ab218419/Images/ab218419-274086-anti-dc-sign-antibody-c209-1781-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)Immunohistochemical analysis of paraffin embedded, formalin fixed human colon carcinoma tissue labeling DC-SIGN using ab218419 at 1 µg/mL.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)](https://www.abcam.com/ps/products/218/ab218419/Images/ab218419-274085-anti-dc-sign-antibody-c209-1781-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)Immunohistochemical analysis of paraffin embedded, formalin fixed human lung carcinoma tissue labeling DC-SIGN using ab218419 at 1 µg/mL.
Datasheets and documents
References (0)
ab218419 has not yet been referenced specifically in any publications.
