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    dc-sign-antibody-c2091781-ab218419.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-DC-SIGN antibody [C209/1781] (ab218419)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)

Key features and details

  • Mouse monoclonal [C209/1781] to DC-SIGN
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2b

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Protein
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Recombinant Human DC-SIGN protein (ab131780)
Secondary
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Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

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Overview

  • Product name

    Anti-DC-SIGN antibody [C209/1781]
    See all DC-SIGN primary antibodies
  • Description

    Mouse monoclonal [C209/1781] to DC-SIGN
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human DC-SIGN. The exact sequence is proprietary.
    Database link: Q9NNX6

  • Positive control

    • IHC-P: Human lung carcinoma, colon carcinoma and small intestine tissues.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    C209/1781
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Microbiology
    • Interspecies Interaction
    • Host Virus Interaction
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • T Cell Adhesion
    • Immunology
    • Immune System Diseases
    • Antiviral Signaling
    • HIV-related

Associated products

  • Alternative Versions

    • Anti-DC-SIGN antibody [C209/1781] - BSA and Azide free (ab218883)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • Recombinant Protein

    • Recombinant Human DC-SIGN protein (ab131780)
  • Related Products

    • Recombinant Human DC-SIGN protein (ab131780)

Applications

Our Abpromise guarantee covers the use of ab218419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
    On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
  • Tissue specificity

    Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
  • Sequence similarities

    Contains 1 C-type lectin domain.
  • Domain

    The tandem repeat domain, also called neck domain, mediates oligomerization.
  • Cellular localization

    Secreted and Cell membrane.
  • Target information above from: UniProt accession Q9NNX6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 30835 Human
    • Omim: 604672 Human
    • SwissProt: Q9NNX6 Human
    • Unigene: 278694 Human
    • Alternative names

      • C type lectin domain family 4 member L antibody
      • C-type lectin domain family 4 member L antibody
      • CD 209 antibody
      • CD209 antibody
      • CD209 antigen antibody
      • CD209 antigen-like protein A antibody
      • CD209 molecule antibody
      • CD209_HUMAN antibody
      • Cd209a antibody
      • CDSIGN antibody
      • CIRE antibody
      • CLEC4L antibody
      • DC SIGN antibody
      • DC SIGN1 antibody
      • DC-SIGN antibody
      • DC-SIGN1 antibody
      • DCSIGN antibody
      • Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody
      • Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody
      • Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody
      • Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody
      • Dengue fever, protection against, included antibody
      • Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody
      • HIV GP120 Binding Protein antibody
      • MGC129965 antibody
      • MGC130443 antibody
      • SIGN-R1 antibody
      • SIGNR5 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)

      Immunohistochemical analysis of paraffin embedded, formalin fixed human small intestine tissue labeling DC-SIGN using ab218419 at 1 µg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)

      Immunohistochemical analysis of paraffin embedded, formalin fixed human colon carcinoma tissue labeling DC-SIGN using ab218419 at 1 µg/mL.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGN antibody [C209/1781] (ab218419)

      Immunohistochemical analysis of paraffin embedded, formalin fixed human lung carcinoma tissue labeling DC-SIGN using ab218419 at 1 µg/mL.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab218419? Please let us know so that we can cite the reference in this datasheet.

    ab218419 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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