Recombinant
RabMAb

Recombinant Anti-DC-SIGN antibody [EPR22395-52] (ab245189)

Overview

  • Product name

    Anti-DC-SIGN antibody [EPR22395-52]
    See all DC-SIGN primary antibodies
  • Description

    Rabbit monoclonal [EPR22395-52] to DC-SIGN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human DC-SIGN aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9NNX6

  • Positive control

    • ICC/IF: THP-1 cells. Flow: THP-1 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab245189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/60.
WB Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.

Not Reccomended

ICC/IF 1/100.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Pathogen-recognition receptor expressed on the surface of immature dendritic cells (DCs) and involved in initiation of primary immune response. Thought to mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. The receptor returns to the cell membrane surface and the pathogen-derived antigens are presented to resting T-cells via MHC class II proteins to initiate the adaptive immune response. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, cytomegalovirus gB, HCV E2, dengue virus gE, Leishmania pifanoi LPG, Lewis-x antigen in Helicobacter pylori LPS, mannose in Klebsiella pneumonae LPS, di-mannose and tri-mannose in Mycobacterium tuberculosis ManLAM and Lewis-x antigen in Schistosoma mansoni SEA.
      On DCs it is a high affinity receptor for ICAM2 and ICAM3 by binding to mannose-like carbohydrates. May act as a DC rolling receptor that mediates transendothelial migration of DC presursors from blood to tissues by binding endothelial ICAM2. Seems to regulate DC-induced T-cell proliferation by binding to ICAM3 on T-cells in the immunological synapse formed between DC and T-cells.
    • Tissue specificity

      Predominantly expressed in dendritic cells and in DC-residing tissues. Also found in placental macrophages, endothelial cells of placental vascular channels, peripheral blood mononuclear cells, and THP-1 monocytes.
    • Sequence similarities

      Contains 1 C-type lectin domain.
    • Domain

      The tandem repeat domain, also called neck domain, mediates oligomerization.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • C type lectin domain family 4 member L antibody
      • C-type lectin domain family 4 member L antibody
      • CD 209 antibody
      • CD209 antibody
      • CD209 antigen antibody
      • CD209 antigen-like protein A antibody
      • CD209 molecule antibody
      • CD209_HUMAN antibody
      • Cd209a antibody
      • CDSIGN antibody
      • CIRE antibody
      • CLEC4L antibody
      • DC SIGN antibody
      • DC SIGN1 antibody
      • DC-SIGN antibody
      • DC-SIGN1 antibody
      • DCSIGN antibody
      • Dendritic cell specific ICAM 3 grabbing nonintegrin 1 antibody
      • Dendritic cell specific ICAM3 grabbing nonintegrin 1 antibody
      • Dendritic cell-specific ICAM-3-grabbing non-integrin 1 antibody
      • Dendritic cell-specific intracellular adhesion molecules (ICAM)-3 grabbing non-integrin antibody
      • Dengue fever, protection against, included antibody
      • Dentritic Cell Specific ICAM3 Grabbing Nonintegrin antibody
      • HIV GP120 Binding Protein antibody
      • MGC129965 antibody
      • MGC130443 antibody
      • SIGN-R1 antibody
      • SIGNR5 antibody
      see all

    Images

    • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 (human monocytic leukemia cell line) cells labeling DC-SIGN using ab245189 at 1/100 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in THP-1 cells treated with 10 ng/ml PMA for 18h, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN expression is induced by PMA plus IL4 in THP-1 (PMID: 15070901; PMID: 22675249). Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) was used to stain tubulin (red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab150077) at 1/1000 dilution.


       

    • Flow cytometric analysis of unfixed non-permeabilized THP-1 (human monocytic leukemia cell line) cells treated with 10 ng/ml PMA for 18 hours, then serum starved for 8 hours, then 10 ng/ml PMA for 6 hours and add 1000 U IL4 for 2 hours, then add 10% serum for another 22 hours. DC-SIGN is labeled with ab245189 at 1/60 dilution in treated (red) and untreated cells (green) as compared to a Rabbit monoclonal IgG (ab172730, black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody, blue).  A Goat anti rabbit IgG (Dylight® 488, ab98462) was used as the secondary antibody at 1/2000 dilution. Gated on viable cells. DC-SIGN expression is induced by PMA plus IL4 in THP-1 (PMID: 15070901; PMID: 22675249).

    • All lanes : Anti-DC-SIGN antibody [EPR22395-52] (ab245189) at 1/1000 dilution

      Lane 1 : Recombinant Human DC-SIGN/CD209 protein (aa59-404) 20 ng
      Lane 2 : Recombinant Human DC-SIGNR/CD299 Fc chimeric protein (aa73-376) 20 ng

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 46 kDa


      Exposure time: 8 seconds


      Blocking/diluting buffer and concentration: 5% NFDM/TBST

    References

    ab245189 has not yet been referenced specifically in any publications.

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