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RecombinantRabMAb

Recombinant Anti-DC-SIGNR antibody [EPR11211] (ab169783)

  • Datasheet
  • SDS
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Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
  • Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
  • Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
  • Anti-DC-SIGNR antibody [EPR11211] (ab169783)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11211] to DC-SIGNR
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-DC-SIGNR antibody [EPR11211]
    See all DC-SIGNR primary antibodies
  • Description

    Rabbit monoclonal [EPR11211] to DC-SIGNR
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Recombinant fragment within Human DC-SIGNR aa 200-400. The exact sequence is proprietary.

  • Positive control

    • WB: Human placenta, Human kidney, and Human liver lysates. IHC-P: human placenta tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR11211
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Anti-DC-SIGNR antibody [EPR11211] - BSA and Azide free (ab240156)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human DC-SIGNR protein (Fc Chimera) (ab83920)

Applications

Our Abpromise guarantee covers the use of ab169783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250

See IHC antigen retrieval protocols.

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus.
    • Tissue specificity

      Predominantly highly expressed in liver sinusoidal endothelial cells and in lymph node. Found in placental endothelium but not in macrophages. Expressed in type II alveolar cells and lung endothelial cells.
    • Sequence similarities

      Contains 1 C-type lectin domain.
    • Domain

      The tandem repeat domain, also called neck domain, mediates oligomerization.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession Q9H2X3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 450160 Chimpanzee
      • Entrez Gene: 101151081 Gorilla
      • Entrez Gene: 10332 Human
      • Omim: 605872 Human
      • SwissProt: Q8HYC0 Chimpanzee
      • SwissProt: Q8HY06 Gorilla
      • SwissProt: Q9H2X3 Human
      • Unigene: 421437 Human
      • Alternative names

        • C type lectin domain family 4, member M antibody
        • C-type lectin domain family 4 member M antibody
        • CD209 antigen like antibody
        • CD209 antigen like protein 1 antibody
        • CD209 antigen-like protein 1 antibody
        • CD209L antibody
        • CD209L1 antibody
        • CD299 antibody
        • CD299 antigen antibody
        • CLC4M_HUMAN antibody
        • CLEC4M antibody
        • DC SIGN related protein antibody
        • DC SIGN2 antibody
        • DC SIGNR antibody
        • DC-SIGN-related protein antibody
        • DC-SIGN2 antibody
        • DC-SIGNR antibody
        • DCSIGN related protein antibody
        • DCSIGNR antibody
        • Dendritic cell-specific ICAM-3-grabbing non-integrin 2 antibody
        • HP10347 antibody
        • L SIGN antibody
        • L-SIGN antibody
        • Liver/lymph node specific ICAM3 grabbing nonintegrin antibody
        • Liver/lymph node-specific ICAM-3-grabbing non-integrin antibody
        • LSIGN antibody
        • Mannose binding C type lectin DC SIGNR antibody
        • MGC129964 antibody
        • MGC47866 antibody
        see all

      Images

      • Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Anti-DC-SIGNR antibody [EPR11211] (ab169783) at 1/10000 dilution (Purified) + Human liver lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 45 kDa
        Observed band size: 45 kDa

      • Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Anti-DC-SIGNR antibody [EPR11211] (ab169783) at 1/10000 dilution (Purified) + Human kidney lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 45 kDa
        Observed band size: 45 kDa

      • Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Anti-DC-SIGNR antibody [EPR11211] (ab169783) at 1/1000 dilution (Purified) + Human placenta lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 45 kDa
        Observed band size: 45 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling DC-SIGNR with purified ab169783 at 1/500 dilution (0.502 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling DC-SIGNR using ab169783 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, labeling DC-SIGNR using unpurified ab169783 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Anti-DC-SIGNR antibody [EPR11211] (ab169783)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (3)

      Publishing research using ab169783? Please let us know so that we can cite the reference in this datasheet.

      ab169783 has been referenced in 3 publications.

      • Xia HB  et al. Decreased DC-SIGNR expression in hepatocellular carcinoma predicts poor patient prognosis. Oncol Lett 19:69-76 (2020). PubMed: 31897116
      • Luo L  et al. High expression levels of CLEC4M indicate poor prognosis in patients with hepatocellular carcinoma. Oncol Lett 19:1711-1720 (2020). PubMed: 32194663
      • Wang X  et al. Overexpression of dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin-related protein in cervical cancer and correlation with squamous cell carcinoma antigen. Oncol Lett 14:2813-2821 (2017). PubMed: 28927040

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

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