Recombinant
RabMAb

Recombinant Anti-DC-SIGNR antibody [EPR11211] (ab169783)

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Overview

  • Product name

    Anti-DC-SIGNR antibody [EPR11211]
    See all DC-SIGNR primary antibodies

  • Description

    Rabbit monoclonal [EPR11211] to DC-SIGNR

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla

  • Immunogen

    Recombinant fragment within Human DC-SIGNR aa 200-400. The exact sequence is proprietary.

  • Positive control

    • WB: Human placenta, Human kidney, and Human liver lysates. IHC-P: human placenta tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR11211

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab169783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250

See IHC antigen retrieval protocols.
  • Application notes
    Is unsuitable for ICC/IF or IP.

    • Target

    • Function

      Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus.

    • Tissue specificity

      Predominantly highly expressed in liver sinusoidal endothelial cells and in lymph node. Found in placental endothelium but not in macrophages. Expressed in type II alveolar cells and lung endothelial cells.

    • Sequence similarities

      Contains 1 C-type lectin domain.

    • Domain

      The tandem repeat domain, also called neck domain, mediates oligomerization.

    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • C type lectin domain family 4, member M antibody
        • C-type lectin domain family 4 member M antibody
        • CD209 antigen like antibody
        • CD209 antigen like protein 1 antibody
        • CD209 antigen-like protein 1 antibody
        • CD209L antibody
        • CD209L1 antibody
        • CD299 antibody
        • CD299 antigen antibody
        • CLC4M_HUMAN antibody
        • CLEC4M antibody
        • DC SIGN related protein antibody
        • DC SIGN2 antibody
        • DC SIGNR antibody
        • DC-SIGN-related protein antibody
        • DC-SIGN2 antibody
        • DC-SIGNR antibody
        • DCSIGN related protein antibody
        • DCSIGNR antibody
        • Dendritic cell-specific ICAM-3-grabbing non-integrin 2 antibody
        • HP10347 antibody
        • L SIGN antibody
        • L-SIGN antibody
        • Liver/lymph node specific ICAM3 grabbing nonintegrin antibody
        • Liver/lymph node-specific ICAM-3-grabbing non-integrin antibody
        • LSIGN antibody
        • Mannose binding C type lectin DC SIGNR antibody
        • MGC129964 antibody
        • MGC47866 antibody
        see all

      Images

      • Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Anti-DC-SIGNR antibody [EPR11211] (ab169783) at 1/10000 dilution (Purified) + Human liver lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 45 kDa
        Observed band size: 45 kDa

      • Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Anti-DC-SIGNR antibody [EPR11211] (ab169783) at 1/10000 dilution (Purified) + Human kidney lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 45 kDa
        Observed band size: 45 kDa

      • Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Western blot - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Anti-DC-SIGNR antibody [EPR11211] (ab169783) at 1/1000 dilution (Purified) + Human placenta lysates at 15 µg

        Secondary
        Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

        Predicted band size: 45 kDa
        Observed band size: 45 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling DC-SIGNR with purified ab169783 at 1/500 dilution (0.502 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling DC-SIGNR using ab169783 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DC-SIGNR antibody [EPR11211] (ab169783)

        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, labeling DC-SIGNR using unpurified ab169783 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • Wang X  et al. Overexpression of dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin-related protein in cervical cancer and correlation with squamous cell carcinoma antigen. Oncol Lett 14:2813-2821 (2017). Read more (PubMed: 28927040) »
      See 1 Publication for this product

      Publishing research using ab169783? Please let us know so that we can cite the reference in this datasheet.

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