Recombinant
RabMAb

Recombinant Anti-DC-SIGNR antibody [EPR11211] - BSA and Azide free (ab240156)

Overview

  • Product name

    Anti-DC-SIGNR antibody [EPR11211] - BSA and Azide free
    See all DC-SIGNR primary antibodies
  • Description

    Rabbit monoclonal [EPR11211] to DC-SIGNR - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Recombinant fragment within Human DC-SIGNR aa 200-400. The exact sequence is proprietary.

  • Positive control

    • WB: Human placenta, Human kidney, and Human liver lysates. IHC-P: human placenta tissue.
  • General notes

    ab240156 is the carrier-free version of ab169783 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240156 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. May mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. Probably recognizes in a calcium-dependent manner high mannose N-linked oligosaccharides in a variety of pathogen antigens, including HIV-1 gp120, HIV-2 gp120, SIV gp120, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S. Is a receptor for ICAM3, probably by binding to mannose-like carbohydrates. Is presumably a coreceptor for the SARS coronavirus.
    • Tissue specificity

      Predominantly highly expressed in liver sinusoidal endothelial cells and in lymph node. Found in placental endothelium but not in macrophages. Expressed in type II alveolar cells and lung endothelial cells.
    • Sequence similarities

      Contains 1 C-type lectin domain.
    • Domain

      The tandem repeat domain, also called neck domain, mediates oligomerization.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • C type lectin domain family 4, member M antibody
      • C-type lectin domain family 4 member M antibody
      • CD209 antigen like antibody
      • CD209 antigen like protein 1 antibody
      • CD209 antigen-like protein 1 antibody
      • CD209L antibody
      • CD209L1 antibody
      • CD299 antibody
      • CD299 antigen antibody
      • CLC4M_HUMAN antibody
      • CLEC4M antibody
      • DC SIGN related protein antibody
      • DC SIGN2 antibody
      • DC SIGNR antibody
      • DC-SIGN-related protein antibody
      • DC-SIGN2 antibody
      • DC-SIGNR antibody
      • DCSIGN related protein antibody
      • DCSIGNR antibody
      • Dendritic cell-specific ICAM-3-grabbing non-integrin 2 antibody
      • HP10347 antibody
      • L SIGN antibody
      • L-SIGN antibody
      • Liver/lymph node specific ICAM3 grabbing nonintegrin antibody
      • Liver/lymph node-specific ICAM-3-grabbing non-integrin antibody
      • LSIGN antibody
      • Mannose binding C type lectin DC SIGNR antibody
      • MGC129964 antibody
      • MGC47866 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue sections labeling DC-SIGNR with purified ab169783 at 1/500 dilution (0.502 µg/ml). Heat mediated antigen retrieval using Bond� Epitope Retrieval Solution 1 (pH 6.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169783)
    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue, labeling DC-SIGNR using ab169783 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169783).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue, labeling DC-SIGNR using ab169783 at a 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab169783).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab240156 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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