Overview

  • Product name

  • Description

    Rabbit polyclonal to DCAMKL1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DCAMKL1 aa 679-729 (C terminal). The exact sequence is proprietary.
    Sequence:

    AA GVSVIALDHG FTIKRSGSLD YYQQPGMYWI RPPLLIRRGR FSDEDATRM


    Database link: O15075-2

  • Positive control

    • Human brain tissue lysate; Rat brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab106635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 82 kDa.

Target

  • Function

    Probable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
  • Tissue specificity

    In fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 2 doublecortin domains.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
    • CL1 antibody
    • CLICK1 antibody
    • Cpg16 antibody
    • DCDC3A antibody
    • Dcl antibody
    • Dclk antibody
    • Dclk1 antibody
    • DCLK1_HUMAN antibody
    • Doublecortin domain-containing protein 3A antibody
    • Doublecortin-like and CAM kinase-like 1 antibody
    • Doublecortin-like kinase 1 antibody
    • KIAA0369 antibody
    • Serine/threonine-protein kinase DCAMKL1 antibody
    • Serine/threonine-protein kinase DCLK1 antibody
    see all

Images

  • Western blot analysis of DCLK1 expression in rat brain tissue lysate with ab106635 at (A) 1 and (B) 2µg/ml.
  • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling DCAMKL1 with Anti-DCAMKL1 antibody (ab106635) at 20μg/ml.
  • ab106635 at 2.5µg/ml, staining DCAMLK1 in Rat brain, using Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.
  • Lane 1 : Anti-DCAMKL1 antibody (ab106635) at 0.5 µg/ml
    Lane 2 : Anti-DCAMKL1 antibody (ab106635) at 1 µg/ml

    All lanes : Human brain tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 82 kDa

References

This product has been referenced in:

  • Sarkar S  et al. A novel antibody against cancer stem cell biomarker, DCLK1-S, is potentially useful for assessing colon cancer risk after screening colonoscopy. Lab Invest 97:1245-1261 (2017). Read more (PubMed: 28414327) »
See 1 Publication for this product

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