Key features and details
- Rabbit polyclonal to DCAMKL1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Product nameAnti-DCAMKL1 antibody
See all DCAMKL1 primary antibodies
DescriptionRabbit polyclonal to DCAMKL1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human, Pig
Predicted to work with: Rat
Recombinant fragment corresponding to Human DCAMKL1 aa 390-647. (Expressed in E.coli).
YKVGRTIGDGNFAVVKECVERSTAREYALKIIKKSKCRGKEHMIQNEVSI LRRVKHPNIVLLIEEMDVPTELYLVMELVKGGDLFDAITSTNKYTERDAS GMLYNLASAIKYLHSLNIVHRDIKPENLLVYEHQDGSKSLKLGDFGLATI VDGPLYTVCGTPTYVAPEIIAETGYGLKVDIWAAGVITYILLCGFPPFRG SGDDQEVLFDQILMGQVDFPSPYWDNVSDSAKELITMMLLVDVDQRFSAV QVLEHPWV
Database link: O15075
- IHC-P: Human prostate, liver, lung cancer, bile duct cancer, stomach cancer and cholangiocarcinoma tissues. WB: Mouse and pig brain lysates; Recombinant human DCAMKL1 protein.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232875 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232875 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionProbable kinase that may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. May also participate in functions of the mature nervous system.
Tissue specificityIn fetal tissues, highly expressed in brain, detectable in lung and liver, but not in kidney. In adult tissues, expressed ubiquitously in the brain, detectable in the heart, liver, spleen, thymus, prostate, testis, ovary, small intestine and colon. The type A isoforms seem to be expressed predominantly in fetal brain whereas type B isoforms are expressed abundantly in both fetal and adult brain.
Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 2 doublecortin domains.
Contains 1 protein kinase domain.
- Information by UniProt
- Calcium/calmodulin-dependent protein kinase type I-like CPG16 antibody
- CL1 antibody
- CLICK1 antibody
Paraffin-embedded human prostate tissue stained for DCAMKL1 using ab232875 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human liver tissue stained for DCAMKL1 using ab232875 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human lung cancer tissue stained for DCAMKL1 using ab232875 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human bile duct cancer tissue stained for DCAMKL1 using ab232875 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human stomach cancer tissue stained for DCAMKL1 using ab232875 at 10 µg/ml in immunohistochemical analysis. DAB staining.
Anti-DCAMKL1 antibody (ab232875) at 3 µg/ml + Recombinant human DCAMKL1 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 82 kDa
All lanes : Anti-DCAMKL1 antibody (ab232875) at 3 µg/ml
Lane 1 : Pig brain lysate
Lane 2 : Mouse brain lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 82 kDa
Paraffin-embedded human cholangiocarcinoma tissue stained for DCAMKL1 using ab232875 at 10 µg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232875 has not yet been referenced specifically in any publications.