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Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse DCAMLK1.
(Peptide available as ab31703.)
Our Abpromise guarantee covers the use of ab31704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|IHC-P||Use at an assay dependent concentration. PubMed: 20522640|
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 47, 82 kDa (predicted molecular weight: 47, 82 kDa).Can be blocked with Mouse DCAMKL1 peptide (ab31703).|
|IP||Use at an assay dependent concentration.|
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: DCAMKL1 knockout HAP1 whole cell lysate (20 µg)
Lane 3: NIH3T3 whole cell lysate (20 µg)
Lane 4: Human brain whole tissue lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab31704 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab31704 was shown to specifically react with DCAMKL1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when DCAMKL1 knockout cells were examined. Wild-type and DCAMKL1 knockout samples were subjected to SDS-PAGE. ab31704 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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