Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13222] to DCBLD1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Product nameAnti-DCBLD1 antibody [EPR13222]
See all DCBLD1 primary antibodies
DescriptionRabbit monoclonal [EPR13222] to DCBLD1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human DCBLD1 aa 250-400. The exact sequence is proprietary.
Database link: Q8N8Z6
- A549, HT1080, NIH OVCAR 3 and SH SY5Y cell line lysates.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab185216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 59 kDa (predicted molecular weight: 78 kDa).|
RelevanceThe specific function of the protein remains unknown.
Cellular localizationMembrane; Single-pass type I membrane protein
- Discoidin, CUB and LCCL domain containing protein 1 antibody
All lanes : Anti-DCBLD1 antibody [EPR13222] (ab185216) at 1/50000 dilution
Lane 1 : A549 cell line lysate
Lane 2 : HT1080 cell line lysate
Lane 3 : NIH OVCAR 3 cell line lysate
Lane 4 : SH SY5Y cell line lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Predicted band size: 78 kDa
ab185216 has been referenced in 1 publication.
- Wang Y et al. SNP rs17079281 decreases lung cancer risk through creating an YY1-binding site to suppress DCBLD1 expression. Oncogene 39:4092-4102 (2020). PubMed: 32231272