Overview

  • Product name

  • Description

    Rabbit polyclonal to DCC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human DCC (internal sequence).
    Database link: P43146

  • Positive control

    • LOVO cells

Properties

Applications

Our Abpromise guarantee covers the use of ab196598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.

Target

  • Function

    Receptor for netrin required for axon guidance. Mediates axon attraction of neuronal growth cones in the developing nervous system upon ligand binding. Its association with UNC5 proteins may trigger signaling for axon repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Implicated as a tumor suppressor gene.
  • Tissue specificity

    Found in axons of the central and peripheral nervous system and in differentiated cell types of the intestine. Not expressed in colorectal tumor cells that lost their capacity to differentiate into mucus producing cells.
  • Involvement in disease

    Defects in DCC are the cause of congenital mirror movements (MIMOC) [MIM:157600]. Mirror movements are contralateral involuntary movements that mirror voluntary ones. While mirror movements are occasionally found in young children, persistence beyond the age of 10 is abnormal. Mirror movements occur more commonly in the upper extremities.
  • Sequence similarities

    Belongs to the immunoglobulin superfamily. DCC family.
    Contains 6 fibronectin type-III domains.
    Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
  • Post-translational
    modifications

    Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Colorectal cancer related chromosome sequence 18 antibody
    • Colorectal cancer suppressor antibody
    • CRC 18 antibody
    • CRC18 antibody
    • CRCR 1 antibody
    • CRCR1 antibody
    • DCC antibody
    • DCC_HUMAN antibody
    • Deleted in colorectal cancer protein antibody
    • Deleted in colorectal carcinoma antibody
    • Immunoglobulin superfamily DCC subclass member 1 antibody
    • Netrin receptor DCC antibody
    • Tumor suppressor protein DCC antibody
    see all

Images

  • Immunofluorescence analysis of LOVO cells labelling DCC using ab196598 at 1/100 dilution. The image on the right is treated with the synthesized peptide.

References

ab196598 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Embryonic Spinal Cord)
Permeabilization
Yes - triton
Specification
Embryonic Spinal Cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 19 2016

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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