Anti-DCC1 antibody (ab168133)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-DCC1 antibody
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DescriptionMouse polyclonal to DCC1
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Host speciesMouse
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Full length protein corresponding to Human DCC1 aa 1-393.
Sequence:MKRTRDEVDATLQIAKLNAAELLPAVHCLGFGPGASGAAAGDFCLLELEP TLCQQLEDGHSLVIRGDKDEQAVLCSKDKTYDLKIADTSNMLLFIPGCKT PDQLKKEDSHCNIIHTEIFGFSNNYWELRRRRPKLKKLKKLLMENPYEGP DSQKEKDSNSSKYTTEDLLDQIQASEEEIMTQLQVLNACKIGGYWRILEF DYEMKLLNHVTQLVDSESWSFGKVPLNTCLQELGPLEPEEMIEHCLKCYG KKYVDEGEVYFELDADKICRAAARMLLQNAVKFNLAEFQEVWQQSVPEGM VTSLDQLKGLALVDRHSRPEIIFLLKVDDLPEDNQERFNSLFSLREKWTE EDIAPYIQDLCGEKQTIGALLTKYSRSSMQNGVKVYNSRRPIS
Database link: NP_076999.1 -
Positive control
- DCC1 transfected 293T cell lysate; A431 cell lysate; HeLa cells.
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General notes
Previously labelled as DSCC1.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.40
Constituent: 99% PBS -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab168133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
Target
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FunctionLoads PCNA onto primed templates regulating velocity, spacing and restart activity of replication forks. May couple DNA replication to sister chromatid cohesion through regulation of the acetylation of the cohesin subunit SMC3.
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Sequence similaritiesBelongs to the DCC1 family.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 79075 Human
- Entrez Gene: 72107 Mouse
- Omim: 613203 Human
- SwissProt: Q9BVC3 Human
- SwissProt: Q14AI0 Mouse
- Unigene: 315167 Human
- Unigene: 277084 Mouse
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Alternative names
- DCC1 antibody
- DCC1_HUMAN antibody
- Defective in sister chromatid cohesion 1 homolog antibody
see all
Images
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Western blot - Anti-DCC1 antibody (ab168133)Anti-DCC1 antibody (ab168133) at 1 µg/ml + A431 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 45 kDa -
Western blot - Anti-DCC1 antibody (ab168133)All lanes : Anti-DCC1 antibody (ab168133) at 1 µg/ml
Lane 1 : DCC1 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
Lane 1 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Lane 2 : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 45 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-DCC1 antibody (ab168133)Immunofluorescence analysis of HeLa cells labeling DCC1 with ab168133 at 10 ug/ml.
Datasheets and documents
References
ab168133 has not yet been referenced specifically in any publications.
