Key features and details
- Rabbit polyclonal to DCD - C-terminal
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DCD antibody - C-terminal
See all DCD primary antibodies
DescriptionRabbit polyclonal to DCD - C-terminal
Tested applicationsSuitable for: WB, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide within Human DCD aa 81-110 (C terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P81605
- T47D cell line lysate, Human skin tissue, 293 cells.
Protein previously labeled as Dermcidin.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityAmmonium Sulphate Precipitation
Purification notesab175519 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Our Abpromise guarantee covers the use of ab175519 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 12 kDa.|
|Flow Cyt||1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionDCD-1 displays antimicrobial activity thereby limiting skin infection by potential pathogens in the first few hours after bacterial colonization. Highly effective against E.coli, E.faecalis, S.aureus and C.albicans. Optimal pH and salt concentration resemble the conditions in sweat.
Survival-promoting peptide promotes survival of neurons and displays phosphatase activity. It may bind IgG.
Tissue specificitySpecifically and constitutively expressed in eccrine sweat gland cells. Secreted into the sweat at a concentration of 1-10 micrograms/ml.
- Information by UniProt
- AIDD antibody
- DCD 1 antibody
- dcd antibody
Immunohistochemical analysis of formalin fixed, paraffin embedded Human skin tissue labeling DCD with ab175519 at 1/50 followed by peroxidase conjugation of the secondary antibody and DAB staining.
Anti-DCD antibody - C-terminal (ab175519) at 1/1000 dilution + T47D cell line lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 12 kDa
Flow cytometric analysis of 293 cells labeling DCD with ab175519 at 1/10 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab175519 has not yet been referenced specifically in any publications.